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Plant Cell Reports

, Volume 28, Issue 6, pp 975–984 | Cite as

Cloning and molecular characterization of fructose-1,6-bisphosphate aldolase gene regulated by high-salinity and drought in Sesuvium portulacastrum

  • Wei Fan
  • Zhili ZhangEmail author
  • Yanlin Zhang
Physiology and Biochemistry

Abstract

Sesuvium portulacastrum, a mangrove plant from seashore, is a halophyte species well adapted to salinity and drought. Some efforts have been made to describe its physiological and structural characteristics on salt and drought-tolerance, but the underlying molecular mechanism and key components have not yet been identified. Here, a fructose-1,6-bisphosphate aldolase gene, designated SpFBA, was isolated and characterized from S. portulacastrum roots in response to seawater. The SpFBA cDNA has a total length of 1452 bp with an open reading frame of 1071 bp, and is predicted to encode a precursor protein of 357 amino acid residues sharing high degree of homology with class I FBAs from other plants. Semi-quantitative RT-PCR analysis indicated that the SpFBA was more strongly expressed in roots than in leaves and stems, and the abiotic stimuli such as Seawater, NaCl, ABA, and PEG, could trigger a significant induction of SpFBA in S. portulacastrum roots within 2–12 h. Overproduction of Recombinant SpFBA resulted in an increased tolerance to salinity in transgenic Escherichia coli. All these results suggest that the SpFBA plays very important roles in responding to salt stress and related abiotic stimuli, and in improving the survival ability of S. portulacastrum under high salinity and drought.

Keywords

Mangrove Sesuvium portulacastrum Seawater Fructose-1,6-bisphosphate aldolase Glycolytic/gluconeogenesis pathway 

Notes

Acknowledgments

This work was supported by the Natural Science Foundation of China (Grant No. 30760197) and the National Nonprofit Institute Research Foundation of CATAS-ITBB (Grant No. ITBBZD0716) in partial coverage of the costs.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouChina
  2. 2.College of Horticulture and LandscapeHainan UniversityDanzhouChina
  3. 3.Hainan Academy of Agricultural SciencesHaikouChina

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