Plant Cell Reports

, Volume 27, Issue 11, pp 1697–1703 | Cite as

Use of SSR markers to determine the anther-derived homozygous lines in coconut

  • P. I. P. Perera
  • L. Perera
  • V. Hocher
  • J. -L. Verdeil
  • D. M. D. Yakandawala
  • L. K. Weerakoon
Cell Biology and Morphogenesis

Abstract

Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs.

Keywords

Coconut Androgenesis Dihaploid Flow cytometry SSR marker 

Abbreviations

BAP

6-Benzylaminopurine

DAPI

4′-6-Diamidino-2-phenylindole

2, 4-D

2, 4-Dichlorophenoxyacetic acid

GA3

Gibberellic acid

SSR

Simple sequence repeats

WBS

Weeks before splitting

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • P. I. P. Perera
    • 1
    • 6
  • L. Perera
    • 2
  • V. Hocher
    • 3
  • J. -L. Verdeil
    • 4
  • D. M. D. Yakandawala
    • 5
    • 6
  • L. K. Weerakoon
    • 1
  1. 1.Tissue Culture DivisionCoconut Research InstituteLunuwilaSri Lanka
  2. 2.Genetics and Plant Breeding DivisionCoconut Research InstituteLunuwilaSri Lanka
  3. 3.Institute for Research and Development (IRD), UMR 1098 BEPC, IRDMontpellier Cedex 1France
  4. 4.CIRADMontpellier Cedex 5France
  5. 5.Department of Botany, Faculty of ScienceUniversity of PeradeniyaPeradeniyaSri Lanka
  6. 6.Post-Graduate Institute of ScienceUniversity of PeradeniyaPeradeniyaSri Lanka

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