Plant Cell Reports

, 27:499 | Cite as

Isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (Fragaria × ananassa Duch.)

  • Yue Ma
  • Haiyue Sun
  • Guiling Zhao
  • Hongyan Dai
  • Xiuyan Gao
  • He Li
  • Zhihong Zhang
Genetic Transformation and Hybridization

Abstract

Strawberry (Fragaria spp.) is a kind of herbaceous perennial plant that propagates vegetatively. The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of LTR retrotransposons were amplified from the cultivated strawberry (Fragaria × ananassa Duch.). Sequence analysis of clones demonstrated that 5 of 19 Ty1-copia group unique sequences and 2 of 10 Ty3-gypsy unique sequences in F. × ananassa genome possessed either stop codon or frameshift. Ty1-copia group sequences are highly heterogeneous (divergence ranged from 1 to 69.8%), but the Ty3-gypsy group sequences are less (divergence ranged from 1 to 10%). Southern dot blot hybridization result suggested that both of the LTR retrotransposons are present in the genome of cultivated strawberry with high copy number (Ty1-copia group 2,875 Ty3-gypsy group 348). RT-PCR amplification from total RNA, which was extracted from leaves of micropropagated strawberry plants, did not yield either of the RT fragments. This is the first report on the presence of RT sequences of Ty1-copia and Ty3-gypsy group retrotransposons in F. × ananassa genome.

Keywords

Fragaria × ananassa Retrotransposons Reverse transcriptase Heterogeneity Transcriptional activity 

Abbreviations

LTR

Long terminal repeat

PCR

Polymerase chain reaction

RT

Reverse transcriptase

TE

Transposable element

RT-PCR

Reverse transcription polymerase chain reaction

S-SAP

Sequence-specific amplification polymorphism

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yue Ma
    • 1
  • Haiyue Sun
    • 1
  • Guiling Zhao
    • 1
    • 3
  • Hongyan Dai
    • 1
  • Xiuyan Gao
    • 1
  • He Li
    • 1
  • Zhihong Zhang
    • 1
    • 2
  1. 1.College of HorticultureShenyang Agricultural UniversityShenyangPeople’s Republic of China
  2. 2.Key Laboratory of Agricultural Biotechnology of Liaoning ProvinceShenyangPeople’s Republic of China
  3. 3.College of ForestryShenyang Agricultural UniversityShenyangPeople’s Republic of China

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