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Plant Cell Reports

, Volume 27, Issue 3, pp 471–478 | Cite as

High-efficiency Agrobacterium-mediated transformation of Brachypodium distachyon inbred line Bd21-3

  • John Vogel
  • Theresa Hill
Genetic Transformation and Hybridization

Abstract

Brachypodium distachyon (Brachypodium) is a small grass with biological attributes (rapid generation time, small genome, diploid accessions, small stature and simple growth requirements) that make it suitable for use as a model system. In addition, a growing list of genomic resources have been developed or are currently under development including: cDNA libraries, BAC libraries, EST sequences, BAC end sequences, a physical map, genetic markers, a linkage map and, most importantly, the complete genome sequence. To maximize the utility of Brachypodium as a model grass it is necessary to develop an efficient Agrobacterium-mediated transformation system. In this report we describe the identification of a transformable inbred diploid line, Bd21-3, and the development of a transformation method with transformation efficiencies as high as 41% of co-cultivated calluses producing transgenic plants. Conducting the co-cultivation step under desiccating conditions produced the greatest improvement in transformation efficiency.

Keywords

Brachypodium Transformation Agrobacterium Biofuel Model system 

Abbreviations

CIM

Callus inducing media

EC

Embryogenic callus

EST

Expressed sequence tag

BAC

Bacterial artificial chromosome

2,4-D

2,4-Dichlorophenoxyacetic acid

LS

Linsmaier and Skoog basal medium

MS

Murashige and Skoog salts and vitamins

Notes

Acknowledgments

We would like to thank David Garvin for seeds of PI 254867, Roger Thilmony for vector pGPro2, James Thomson for vectors p#1, p#4, p#6 and Naxin Huo for technical assistance. This work was supported by USDA CRIS project 5325-21000-013-00 “Biotechnological Enhancement of Energy Crops.”

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.USDA Western Regional Research CenterAlbanyUSA

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