Plant Cell Reports

, Volume 27, Issue 2, pp 297–305

Improvement of Agrobacterium-mediated transformation in Hi-II maize (Zea mays) using standard binary vectors

  • Juan M. Vega
  • Weichang Yu
  • Angela R. Kennon
  • Xinlu Chen
  • Zhanyuan J. Zhang
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-007-0463-z

Cite this article as:
Vega, J.M., Yu, W., Kennon, A.R. et al. Plant Cell Rep (2008) 27: 297. doi:10.1007/s00299-007-0463-z

Abstract

High-frequency transformation of maize (Zea mays L.) using standard binary vectors is advantageous for functional genomics and other genetic engineering studies. Recent advances in Agrobacterium tumefaciens-mediated transformation of maize have made it possible for the public to transform maize using standard binary vectors without a need of the superbinary vector. While maize Hi-II has been a preferred maize genotype to use in various maize transformation efforts, there is still potential and need in further improving its transformation frequency. Here we report the enhanced Agrobacterium-mediated transformation of immature zygotic embryos of maize Hi-II using standard binary vectors. This improved transformation process employs low-salt media in combined use with antioxidant l-cysteine alone or l-cysteine and dithiothreitol (DTT) during the Agrobacterium infection stage. Three levels of N6 medium salts, 10, 50, and 100%, were tested. Both 10 and 50% salts were found to enhance the T-DNA transfer in Hi-II. Addition of DTT to the cocultivation medium also improves the T-DNA transformation. About 12% overall and the highest average of 18% transformation frequencies were achieved from a large number of experiments using immature embryos grown in various seasons. The enhanced transformation protocol established here will be advantageous for maize genetic engineering studies including transformation-based functional genomics.

Keywords

Agrobacterium tumefaciens Binary vector Hi-II maize Zea mays Transformation 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Juan M. Vega
    • 1
  • Weichang Yu
    • 2
  • Angela R. Kennon
    • 3
  • Xinlu Chen
    • 3
  • Zhanyuan J. Zhang
    • 3
  1. 1.Departamento de Genetica, Facultad de BiologiaUniversidad ComplutenseMadridSpain
  2. 2.Division of Biological Science, College of Art and ScienceUniversity of MissouriColumbiaUSA
  3. 3.Plant Transformation Core Facility, Division of Plant Sciences, College of Agriculture, Food and Natural ResourcesUniversity of MissouriColumbiaUSA

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