Plant Cell Reports

, Volume 27, Issue 2, pp 377–385

The involvement of a P38-like MAP kinase in ABA-induced and H2O2-mediated stomatal closure in Vicia faba L.

  • Jing Jiang
  • Pengtao Wang
  • Guoyong An
  • Pengcheng Wang
  • Chun-Peng Song
Biotic and Abiotic Stress

Abstract

SB203580 is a specific inhibitor of p38 mitogen-activated protein (MAP) kinase and has been widely used to investigate the physiological roles of p38 in animal and yeast cells. Here by using an epidermal strip bioassay, laser-scanning confocal microscopy and whole-cell patch clamp analysis, we assess the effects of pyridinyl imidazoles-like SB203580 on the H2O2 signaling in guard cells of Vicia faba L. The results indicated that SB203580 blocks H2O2- or ABA-induced stomatal closure, ABA-induced H2O2 generation, and decrease in K+ fluxing across plasma membrane of Vicia guard cells by application of ABA and H2O2, whereas its analog SB202474 had no effect on these events. Thus, these results suggest that activation of p38-like MAP kinase modulates guard cell ROS signaling in response to stress.

Keywords

H2O2 ABA SB203580 Guard cell 

Abbreviations

ABA

Abscisic acid

ABI

ABA insensitive

AtMPK

Arabidopsis thaliana MAP Kinase

H2DCF-DA

Dichlorofluorescin diacetate

H2O2

Hydrogen peroxide

MBP

Myelin basic protein

MAP kinase

Mitogen-activated protein kinase

ROS

Reactive oxygen species

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jing Jiang
    • 1
  • Pengtao Wang
    • 1
  • Guoyong An
    • 1
  • Pengcheng Wang
    • 1
  • Chun-Peng Song
    • 1
  1. 1.Laboratory of Plant Stress Biology and College of Life SciencesHenan UniversityKaifengPeople’s Republic of China

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