Analysis of molecular markers in three different tomato cultivars exposed to ozone stress
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Three differentially expressed cDNAs have been isolated from ozone treated tomato seedlings. Their level of expression after ozone exposure has been analysed in three tomato cultivars with different sensitivity to ozone (Nikita, Alisa Craig and Valenciano). These comparative analyses have been extended to a number of genes involved in antioxidative, wounding or pathogenesis responses, showing several differences among cultivars that could be related with their different sensitivity to ozone. Gene response to ozone was affected not only by the period and dose of ozone exposure (short time or chronic), but also by growth conditions (controlled growth chamber or field). Comparison of gene expression patterns puts on evidence the needing of validation in field of experiments performed with plants grown under controlled conditions. Our results suggest that changes in genes expression, observed after ozone treatment in field, are affected by additional factors related to environmental clues.
KeywordsEnvironmental stress Oxidative stress Ozone Stress responses Tomato cultivars
This article is based on a study that formed part of the European project TOMSTRESS (engineering tomato against environmental stress FAIR5-CT97-3493). It was also funded by Spain govern in Comisión nacional de ciencia y tecnologia (AGF1998-1600-CE). Our special thanks to Generalitat Valenciana and Bancaixa for their continuous support to Fundación CEAM.
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