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Plant Cell Reports

, Volume 27, Issue 1, pp 197–207 | Cite as

Analysis of molecular markers in three different tomato cultivars exposed to ozone stress

  • F. Marco
  • E. Calvo
  • P. CarrascoEmail author
  • M. J. Sanz
Biotic and Abiotic Stress

Abstract

Three differentially expressed cDNAs have been isolated from ozone treated tomato seedlings. Their level of expression after ozone exposure has been analysed in three tomato cultivars with different sensitivity to ozone (Nikita, Alisa Craig and Valenciano). These comparative analyses have been extended to a number of genes involved in antioxidative, wounding or pathogenesis responses, showing several differences among cultivars that could be related with their different sensitivity to ozone. Gene response to ozone was affected not only by the period and dose of ozone exposure (short time or chronic), but also by growth conditions (controlled growth chamber or field). Comparison of gene expression patterns puts on evidence the needing of validation in field of experiments performed with plants grown under controlled conditions. Our results suggest that changes in genes expression, observed after ozone treatment in field, are affected by additional factors related to environmental clues.

Keywords

Environmental stress Oxidative stress Ozone Stress responses Tomato cultivars 

Notes

Acknowledgments

This article is based on a study that formed part of the European project TOMSTRESS (engineering tomato against environmental stress FAIR5-CT97-3493). It was also funded by Spain govern in Comisión nacional de ciencia y tecnologia (AGF1998-1600-CE). Our special thanks to Generalitat Valenciana and Bancaixa for their continuous support to Fundación CEAM.

References

  1. Bae GY, Nakajima N, Ishizuka K, Kondo N (1996) The role in ozone phytotoxicity of the evolution of ethylene upon induction of 1-aminocyclopropane-1-carboxylic acid synthase by ozone fumigation in tomato plants. Plant Cell Physiol 37:129–134Google Scholar
  2. Bahl A, Loitsch SM, Kahl G (1995) Transcriptional activation of plant defence genes by short-term air pollutant stress. Environ Pollut 89:221–227PubMedCrossRefGoogle Scholar
  3. Baier A, Kandlbinder A, Golldack D, Dietz KJ (2005) Oxidative stress and ozone, perception, signalling and response. Plant Cell Environ 28:1012–1020CrossRefGoogle Scholar
  4. Bytnerowicz A, Arbaugh MJ, Alonso R (2003) Ozone air pollution in the Sierra Nevada: distribution and effects on forests. In: Krupa SV (ed) Developments in environmental science 2, Elsevier, Amsterdam, pp 402Google Scholar
  5. Buchanan-Wollaston V, Earl S, Harrison E, Mathas E, Navabpour S, Page T, Pink D (2003) The molecular analysis of leaf senescence: a genomics approach. Plant Biotechnol J 1:3–22PubMedCrossRefGoogle Scholar
  6. Conklin PL, Last R (1995) Differential accumulation of antioxidant mRNAs in Arabidopsis thaliana exposed to ozone. Plant Physiol 109:203–212PubMedCrossRefGoogle Scholar
  7. Craker L (1971) Ethylene production from ozone injured plants. Environ Pollut 1:299–304CrossRefGoogle Scholar
  8. Debeaujon I, Peeters AJM, Léon-Kloosterziel KM, Koornneef M (2001) The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium. Plant Cell 13:853–871PubMedCrossRefGoogle Scholar
  9. Eckardt NA (2001) Move it on out with MATEs. Plant Cell 13:1477–1480PubMedCrossRefGoogle Scholar
  10. Elvira S, Alonso R, Castillo F, Gimeno BS (1998) On the response of pigments and antioxidants of Pinus halepensis seedlings to Mediterranean climatic factors and long-term ozone exposure. New Phytol 138:419–432CrossRefGoogle Scholar
  11. Guidi L, Degl’Innocenti E, Genovesi S, Soldatini GF (2005) Photosynthetic process and activities of enzymes involved in the phenylpropanoid pathway in resistant and sensitive genotypes of Lycopersicon esculentum L. exposed to ozone. Plant Sci 168:153–160CrossRefGoogle Scholar
  12. Gunthardt-Goerg MS, Matyssek R, Scheidegger C, Keller T (1993) Differentiation and structural decline in the leaves and bark of birch (Betula pendula) under low ozone concentrations. Trees Struct Funct 7:104–114Google Scholar
  13. Halperin T, Zheng B, Itzhaki H, Clarke AK, Adam Z (2001) Plant mitochondria contain proteolytic and regulatory subunits of the ATP-dependent Clp protease. Plant Mol Biol 45:461–468PubMedCrossRefGoogle Scholar
  14. Hara K, Yagi M, Koizumi N, Kusano T, Sano H (2000) Screening of wound-responsive genes identifies an immediate-early expressed gene encoding a highly charged protein in mechanically wounded tobacco plants. Plant Cell Physiol 41:684–691PubMedCrossRefGoogle Scholar
  15. Heath RL (1994) Possible mechanisms for the inhibition of photosynthesis by ozone. Photosynth Res 39:439–451CrossRefGoogle Scholar
  16. Krupa SV (1996) The role of atmospheric chemistry in the assessment of crop growth and productivity. In: Yumus M, Iqbal M (eds) Plant response to air pollution, Wiley, London, pp 35–74Google Scholar
  17. Lefohn AS (1992) Surface level ozone exposure and their effects on vegetation. Lewis, London, p 366Google Scholar
  18. Liang P, Pardee AB (1998) Differential display. A general protocol. Mol Biotechnol 10:261–267PubMedCrossRefGoogle Scholar
  19. Lim PO, Woo HR, Nam HG (2003) Molecular genetics of leaf senescence in Arabidopsis. Trends Plant Sci 8:272–278PubMedCrossRefGoogle Scholar
  20. Maccarrone M, Veldink GA, Vliegenhardt JFG (1992) Thermal injury and ozone stress affect soybean lipoxygenase expression. FEBS Lett 309:225–230PubMedCrossRefGoogle Scholar
  21. Matsuyama T, Tamaoki M, Nakajima N, Aono M, Kubo A, Moriya S, Ichihara T, Suzuki O, Saji H (2002) cDNA microarray assessment for ozone-stressed Arabidopsis thaliana. Environ Pollut 117:191–4PubMedCrossRefGoogle Scholar
  22. Matyssek R, Sandermann H (2003) Impact of ozone on trees: an ecophysiological perspective. Progress in botany 64. Springer, Heidelberg, pp 349–404Google Scholar
  23. McCrady JK, Andersen CP (2000) The effect of ozone on below-ground carbon allocation in wheat. Environ Pollut 107:465–472PubMedCrossRefGoogle Scholar
  24. Miller JD, Arteca RN, Pell EJ (1999) Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis. Plant Physiol 120:1015–1024PubMedCrossRefGoogle Scholar
  25. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498PubMedCrossRefGoogle Scholar
  26. Miyazaki S, Fredricksen M, Hollis KC, Poroyko V, Shepley D, Galbaith DW, Long SP, Bohnert HJ (2004) Transcript expression profiles of Arabidopsis thaliana grown under controlled conditions and open-air elevated concentrations of CO2 and O3. Field Crop Res 90:47–59CrossRefGoogle Scholar
  27. Ojanperä K, Pättsikkä E, Ylarantä E (1998) Effects of low ozone exposure of spring wheat on net CO2 uptake, rubisco, leaf senescence and grain filling. New Phytol 138:451–460CrossRefGoogle Scholar
  28. Pell EJ, Sinn JP, Eckardt N, Vinten-Johansen C, Winner WE, Mooney HA (1993) Response of radish to multiple stresses II. Influence of season and genotype on plant response to ozone and soil moisture deficit. New Phytol 115:439–446CrossRefGoogle Scholar
  29. Pell EJ, Schlagnhaufer CD, Arteca RN (1997) Ozone induced oxidative stress: mechanism of action and reaction. Physiol Plantarum 100:264–273CrossRefGoogle Scholar
  30. Rao MV, Davis KR (2001) The physiology of ozone induced cell death. Planta 213:682–690PubMedCrossRefGoogle Scholar
  31. Ruzsa SM, Mylona P, Scandalios JG (1999) Differential response of antioxidant genes in maize leaves exposed to ozone. Redox Report 4:95–103PubMedCrossRefGoogle Scholar
  32. Reiling K, Davison AW (1995) Effects of ozone on stomatal conductance and photosynthesis in populations of Plantago major L. New Phytol 129:587–594CrossRefGoogle Scholar
  33. Saitanis CJ, Karandinos MG (2002) Effects of ozone on tobacco (Nicotiana tabacum L) varieties. J Agron Crop Sci 188:51–58CrossRefGoogle Scholar
  34. Salam MA, Soja G (1995) Bush bean (Phaseolus vulgaris L) leaf injury, photosynthesis and stomatal functions under elevated ozone levels. Water Air Soil Pollut 85:1533–1538CrossRefGoogle Scholar
  35. Sandermann H Jr (1996) Ozone and plant health. Annu Rev Phytopathology 34:347–66CrossRefGoogle Scholar
  36. Sandermann H Jr (1998) Ozone: an air pollutant acting as a plant-signaling molecule. Naturwissenschaften 85:369–75CrossRefGoogle Scholar
  37. Sanz MJ, Millán MM (2000) Ozone in the Mediterranean region: Evidence of injury to vegetation. In: Innes JL, Oleskyn J (eds) Forest dynamics in heavily polluted regions. CAB International, London, pp 165–192Google Scholar
  38. Sävenstrand H, Brosche M, Aengehagen M, Strid A (2000) Molecular markers for ozone stress isolated by suppression subtractive hybridization: specificity of gene expression and identification of a novel stress-regulated gene. Plant Cell Environ 23:689–700CrossRefGoogle Scholar
  39. Schraudner MD, Ernst D, Langebartels C, Sandermann H (1992) Biochemical plant response to ozone. III. Activation of defence-related proteins b-1,3-glucanase and chitinase in tobacco leaves. Plant Physiol 99:1321–1328PubMedCrossRefGoogle Scholar
  40. Sharma YK, Davis KR (1994) Ozone-induced expression of stress-related genes in Arabidopsis thaliana. Plant Physiol 105:1089–1096PubMedGoogle Scholar
  41. Sharma YK, Davis KR (1997) The effects of ozone on antioxidant responses in plants. Free Radic Biol Med 23:480–488PubMedCrossRefGoogle Scholar
  42. Shirley BW (1996) Flavonoid biosynthesis: ‘new’ functions for an ‘old’ pathway. Trends Plant Sci 1:377–382CrossRefGoogle Scholar
  43. Tamaoki M, Nakajima N, Kubo A, Aono M, Matsuyama T, Saji H (2003a) Transcriptome analysis of O3-exposed Arabidopsis reveals that multiple signal pathways act mutually antagonistically to induce gene expression. Plant Mol Biol 53:443–456PubMedCrossRefGoogle Scholar
  44. Tamaoki M, Matsuyama T, Kanna M, Nakajima N, Kubo A, Aono M, Saji H (2003b) Differential ozone sensitivity among Arabidopsis accessions and its relevance to ethylene synthesis. Planta 216:552–60PubMedGoogle Scholar
  45. Tingey DT, Standley C, Field RW (1976) Stress ethylene evolution: a measure of ozone effects on plants. Atmos Environ 10:969–974PubMedCrossRefGoogle Scholar
  46. US EPA (1996) Air quality criteria for ozone and other photochemical oxidants. National Center for Environmental Assessment, Research Triangle Park, NCGoogle Scholar
  47. Willekens H, Van Camp W, Van Montagu M, Inze D, Langebartels C, Sandermann H Jr (1994) Ozone, sulfur dioxide, and ultraviolet B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L. Plant Physiol 106:1007–1014PubMedGoogle Scholar
  48. Winner WE (1994) Mechanistic analysis of plant responses to air pollution. Ecol Appl 4:651–661CrossRefGoogle Scholar
  49. Zheng Y, Stevenson KJ, Barrowclife R, Chen S, Wang H, Barnes JD (1998) Ozone levels in Chongquing: a potential threat to crop plants commonly grown in the region?. Environ Pollut 99:299–308PubMedCrossRefGoogle Scholar
  50. Zheng Y, Shimizu H, Barnes JD (2002) Limitations to CO2 assimilation in ozone-exposed leaves of Plantago major. New Phytol 155:67–78CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Fundación Centro Estudios Ambientales del Mediterráneo, Parque TecnológicoPaterna ValenciaSpain
  2. 2.Departament de Bioquímica i Biologia Molecular, Facultat de Ciències BiològiquesUniversitat de ValènciaBurjassot ValenciaSpain

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