Plant Cell Reports

, Volume 26, Issue 10, pp 1727–1732

Induction and origin of adventitious shoots from chimeras of Brassica juncea and Brassica oleracea

  • Xue-Yun Zhu
  • Man Zhao
  • Sheng Ma
  • Ya-Ming Ge
  • Ming-Fang Zhang
  • Li-Ping Chen
Cell Biology and Morphogenesis


The chimeras between tuber mustard (Brassica juncea) and red cabbage (B. oleracea) were artificially synthesized in our previous study. Adventitious shoots were induced from nodal segments and leaf discs of TCC (LI-LII-LIII, LI -the outmost layer of shoot apical meristem; LII -the middle layer; LIII -the innermost layer. T = Tuber mustard, C = Red cabbage) chimeras. The origin of the shoots was analyzed by histology and molecular biology. As a result, the frequency of adventitious shoot induction rose with the increase of BA in MS medium in the area of the nodes. However, there was no different induction frequency of adventitious shoots from nodal segment bases in media with different BA concentrations. Most adventitious shoots (clustered shoots) arising from the node area were TTT (Tuber mustard- Tuber mustard- Tuber mustard) and only 4 shoots were chimeras, which indicated that more shoots originated from LI than from LII and LIII. All shoots from nodal segment bases were CCC (Red cabbage-Red cabbage- Red cabbage), indicating that the shoots originated from LII or LII and LIII. There were significant differences in the regeneration rate in the margin of the leaf discs among the three combinations of BA and NAA. Most adventitious shoots from the margin of leaf discs were CCC but 2 out of 70 were chimeras, which indicated that more shoots originated from LII or LII and LIII than from LI. All chimeras obtained by regeneration were different from the original explant donor in type in the present study. The origin of the adventitious shoots varied with the site of origin on the donor plant, and could be multicellular and multihistogenic.


Brassica juncea Brassica oleracea Chimera Adventitious shoot Origin 



6-Benzyladenine acid


α-Naphthaleneacetic acid


Murashige and Skoog medium


Leaf margin-induced adventitious shoots


Leaf vein-induced adventitious shoots


Nodal base-induced adventitious shoots


Nodal-induced adventitious shoots


  1. Balkema GH (1972) Diplontic drift in chimeric plants. Rad Bot 12:51–55CrossRefGoogle Scholar
  2. Broertjes C, Keen A (1980) Adventitious shoots: do they develop from one cell? Euphytica 29:73–87CrossRefGoogle Scholar
  3. Broertjes C, Van Harten AM (1978) Application of mutation breeding methods in the improement of vegetatively propagated crops. Elsevier, New YorkGoogle Scholar
  4. Burk LG (1975) Clonal and selective propagation of tobacco from leaves. Plant Sci Lett 4:149–154CrossRefGoogle Scholar
  5. Chen LP, Ge YM, Zhu XY (2006) Artificial synthesis of interspecific chimeras between tuber mustard (Brassica juncea) and cabbage (Brassica oleracea) and cytological analysis. Plant Cell Rep 25(9):907–913PubMedCrossRefGoogle Scholar
  6. Dermen H (1948) Periclinal cytochimeras and origin of tissues in stem and leaf of peach. Am J Bot 40:154–168CrossRefGoogle Scholar
  7. Dulieu H (1967) Étude de la stabilité.d’une deficience chlorophylienne induite chez le tabac par traitement au methane sulfonate d’éthyle. Ann Amelior Plantes 17:339–355Google Scholar
  8. Klekowski EJ, Kazarinova-Fukshansky N (1984) Shoot apical meristems and mutation: fixation of selectively neutral cell genotypes. Am J Bot 71:22–27CrossRefGoogle Scholar
  9. Li MY (2005) Observation of high-frequency occurrence of chimeral adventitious shoots in tissue culture from the chimeral tissues of Pelargonium zonale. Hort Sci 40(5):1461–1463Google Scholar
  10. Marcotrigiano M (1984) Experimentally synthesized plant chimeras 1. In vitro recovery of Nicotiana tabacum L. chimeras from mixed callus cultures. Ann Bot 54:503–511Google Scholar
  11. Marcotrigiano M (1986a) Origin of adventitious shoots regenerated from cultured tobacco leaf tissue. Am J Bot 73(11):1541–1547CrossRefGoogle Scholar
  12. Marcotrigiano M (1986b) Experimentally synthesied plant chimeras 3. Qualitative and quantitative characteristics of the flowers of interspecific nicotiana chimeras. Ann Bot 57:435–442Google Scholar
  13. Marcotrigiano M (1990) Genetic mosaics and chimeras: implications in biotechnology. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 11. Somaclonal variation and crop improvement. Springer, Berlin, pp 85–111Google Scholar
  14. Miedema P (1973) The use of adventitious buds to prevent chimerism in mutation breeding of potato. Euphytica 22:209–219CrossRefGoogle Scholar
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473–497CrossRefGoogle Scholar
  16. Norris R, Smith R H, Vaughn K C (1983) Plant chimeras used to establish de novo origin of shoots. Science 220:75–76PubMedCrossRefGoogle Scholar
  17. Stewart RN, Derme H (1970) Somatic genetic analysis of the apical layers of chimeral sports in Chrysunthemum by experimental production of adventitious shoots. Am J Bot 57:1061–1071CrossRefGoogle Scholar
  18. Tian HC, Marcotrigiano M (1993) Origin and development of adventitious shoot meristems initiated on plant chimeras. Dev Biol 155:259–269PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Xue-Yun Zhu
    • 1
  • Man Zhao
    • 1
  • Sheng Ma
    • 1
  • Ya-Ming Ge
    • 1
  • Ming-Fang Zhang
    • 1
  • Li-Ping Chen
    • 1
  1. 1.Department of Horticulture, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina

Personalised recommendations