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Plant Cell Reports

, Volume 26, Issue 8, pp 1275–1282 | Cite as

Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato

  • Babak Behnam
  • Akira Kikuchi
  • Fevziye Celebi-Toprak
  • Mie Kasuga
  • Kazuko Yamaguchi-Shinozaki
  • Kazuo N. Watanabe
Genetic Transformation and Hybridization

Abstract

The freezing tolerance of 38 independent transgenic potato lines derived from the cultivar Desiree was tested in vitro using plantlets. The lines were transgenic for the DREB1A gene under control of the rd29A promoter, both of which were derived from Arabidopsis thaliana. The level of damage caused by freezing varied significantly among the transgenic clones and a non-transgenic control (cv. Desiree). Phenotypic evaluation indicated that the variable responses to freezing were attributable to genotypic variation, but freezing tolerance was not dependent on the number of insertions. Northern blot analysis using a DREB1A cDNA probe revealed high levels of DREB1A expression among the transgenic clones during the initial cold exposure at 4°C (after 2 h) and in the early stages of freezing (−20°C, 1–10 min). Furthermore, a linear correlation was detected between the level of expression and the phenotypic response for all lines except D138. Thus, in the case of potato, a significant increase in freezing tolerance was observed in vitro on a small scale following the introduction of rd29A::DREB1A. Additional testing will show whether this strategy can be used for tolerance breeding in potato and to increase the freezing tolerance of other agriculturally important crops.

Keywords

Potato Freezing tolerance rd29A::DREB1A Arabidopsis Transgenic 

Abbreviations

ANOVA

Analysis of variance

CBF

C-repeat-binding factor

CRT

C-repeat

DRE

Dehydration-responsive element

DREB

Dehydration-responsive element binding

DSC

Non-transgenic cv. Desiree as control

r

Correlation coefficient

RCBD

Randomized complete block design

rd29A

Responsive drought 29A

RT

Room temperature

Notes

Acknowledgments

This research was supported by the Life Science Program of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, Grant “S” no. H16-1007 from the University of Tsukuba, and a Grant-in-Aid (Kiban A no.17208001) from the Japan Society for the Promotion of Sciences.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Babak Behnam
    • 1
  • Akira Kikuchi
    • 1
  • Fevziye Celebi-Toprak
    • 2
  • Mie Kasuga
    • 3
  • Kazuko Yamaguchi-Shinozaki
    • 3
  • Kazuo N. Watanabe
    • 1
  1. 1.Gene Research Center, Graduate School of Life and Environmental SciencesUniversity of TsukubaIbarakiJapan
  2. 2.Department of Biology, Division of Molecular BiologyPamukkale UniversityDenizliTurkey
  3. 3.Japan International Research Center for Agricultural Sciences (JIRCAS)TsukubaJapan

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