Plant Cell Reports

, Volume 26, Issue 6, pp 791–804 | Cite as

Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight

  • S. Maruthasalam
  • K. Kalpana
  • K. K. Kumar
  • M. Loganathan
  • K. Poovannan
  • J. A. J. Raja
  • E. Kokiladevi
  • R. Samiyappan
  • D. Sudhakar
  • P. Balasubramanian
Genetic Transformation and Hybridization

Abstract

Elite indica rice cultivars were cotransformed with genes expressing a rice chitinase (chi11) and a thaumatin-like protein (tlp) conferring resistance to fungal pathogens and a serine-threonine kinase (Xa21) conferring bacterial blight resistance, through particle bombardment, with a view to pyramiding sheath blight and bacterial blight resistance. Molecular analyses of putative transgenic lines by polymerase chain reaction, Southern Blot hybridization, and Western Blotting revealed stable integration and expression of the transgenes in a few independent transgenic lines. Progeny analyses showed the stable inheritance of transgenes to their progeny. Coexpression of chitinase and thaumatin-like protein in the progenies of a transgenic Pusa Basmati1 line revealed an enhanced resistance to the sheath blight pathogen, Rhizoctonia solani, as compared to that in the lines expressing the individual genes. A transgenic Pusa Basmati1 line pyramided with chi11, tlp, and Xa21 showed an enhanced resistance to both sheath blight and bacterial blight.

Keywords

Bacterial blight Chitinase Disease resistance Sheath blight Thaumatin-like protein 

Abbreviations

BB

bacterial blight

FRI

functional resistance/susceptibility index

HRLH%

highest relative lesion height %

PB1

Pusa Basmati1

ShB

sheath blight

Notes

Acknowledgements

The authors are grateful to The Rockefeller Foundation, USA [# 99001/225 (PB) and # 99001/227 (DS)] and the Department of Biotechnology, New Delhi, India, for providing the financial support. We greatly acknowledge the help provided by Dr. Legrand, Strasbourg, Cedex, France and Dr. S. Muthukrishnan, Kansas State University, USA.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • S. Maruthasalam
    • 1
  • K. Kalpana
    • 1
  • K. K. Kumar
    • 1
  • M. Loganathan
    • 1
  • K. Poovannan
    • 1
  • J. A. J. Raja
    • 1
  • E. Kokiladevi
    • 1
  • R. Samiyappan
    • 2
  • D. Sudhakar
    • 1
  • P. Balasubramanian
    • 1
  1. 1.Rice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and BiotechnologyTamil Nadu Agricultural UniversityCoimbatoreIndia
  2. 2.Center for Plant Protection Studies, Department of Plant PathologyTamil Nadu Agricultural UniversityCoimbatoreIndia

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