Plant Cell Reports

, Volume 26, Issue 5, pp 693–700 | Cite as

In vitro screening of potato against water-stress mediated through sorbitol and polyethylene glycol

  • Jai Gopal
  • Kazuto Iwama
Biotic and Abiotic Stress


With the objective to develop a practical and effective method of screening potato for drought tolerance, shoot and root growth in microtuber-derived plantlets was studied in vitro in three genotypes with known root mass production under field conditions. Different levels of water-stress were induced using five concentrations of either sorbitol or polyethylene glycol (PEG) in MS medium. Water potential of various media ranged from −0.80 MPa to −2.05 MPa. Water-stress in culture adversely affected plantlet growth, and genotypes differed for their responses. Genotype IWA-1 was less affected than IWA-3 and IWA-5. At the same level of water potential, sorbitol had lower adverse effect than PEG; the latter being sticky. Genotype × sorbitol and genotype × PEG interactions were significant. At 0.2 M sorbitol and 0.003 M PEG, IWA-1 had significantly more roots with higher total root length, root volume, as well as root-dry weight than those of IWA-3 and IWA-5, whereas the latter two genotypes were at par for all these characters. This pattern was similar to the reported pattern of these genotypes for root-dry weight under field conditions. It is concluded that in vitro screening of potato under specific and limited water-stress conditions may provide a system for effectively differentiating the genotypes for their expected root mass production under field conditions.


Drought tolerance In vitro rooting Micropropagation Solanum tuberosum 



Polyethylene glycol


Murashige and Skoog (1962)



Dr. Jai Gopal is thankful to the Japan Society for Promotion of Science (JSPS) for the award of Invitation Fellowship, and to the Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan for inviting him as Visiting Professor. We are thankful to Dr. Y. Jitsuyama for help in arranging the facilities, and to Mr. Suita, Ms. Dunia, and Mrs. Katayama for their kind help in undertaking this study. We are also thankful to Mr. Noboru Ohnishi of Kirin Brewery Co. Ltd., Japan for supplying the microtubers.


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Crop Science Laboratory, Research Faculty of AgricultureHokkaido UniversitySapporoJapan
  2. 2.Division of Crop ImprovementCentral Potato Research InstituteShimlaIndia

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