Plant Cell Reports

, Volume 26, Issue 3, pp 365–371

Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.

Physiology and Biochemistry


Shoot, root, and callus cultures of Scrophularia nodosa L. (Scrophulariaceae) were established and cultivated in vitro. Iridoid glycosides, such as harpagoside, aucubin, and catalpol were identified by LC-ESI-MS and their contents determined by HPLC. For comparison intact plants of S. nodosa were analysed. In shoot cultures slightly lower amounts of detectable iridoid glycosides (4.36% dry weight) were determined than in the field grown plants (4.88%). Concentration of harpagoside was highest in leaves of field plants (1.05%) and in flowers of in vitro plantlets (1.10%). For aucubin the highest amount was found in the leaves of in vitro plantlets (1.67%) whereas the levels of aucubin in the leaves of field plants were remarkably lower. Catalpol was produced as a trace compound in intact plants and shoot cultures. Callus and root cultures were apparently not able to synthesise iridoid glycosides.


Scrophularia nodosa Iridoid glycosides Harpagoside Aucubin Plant in vitro culture 



Woody plant medium (Lloyd and McCown 1980)


Gibberellic acid


2,4-dichloroacetic acid


High performance liquid chromatography


Liquid chromatography – electrospray ionisation – mass spectrometry


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Institut für Pharmazie und Molekulare BiotechnologieUniversität HeidelbergHeidelbergGermany

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