Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature
Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic potato plants with enhanced tolerance to environmental stress, the genes of both Cu/Zn superoxide dismutase and ascorbate peroxidase were expressed in chloroplasts under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SSA plants). SSA plants showed enhanced tolerance to 250 μM methyl viologen, and visible damage in SSA plants was one-fourth that of non-transgenic (NT) plants that were almost destroyed. In addition, when SSA plants were treated with a high temperature of 42°C for 20 h, the photosynthetic activity of SSA plants decreased by only 6%, whereas that of NT plants decreased by 29%. These results suggest that the manipulation of the antioxidative mechanism of the chloroplasts may be applied in the development of industrial transgenic crop plants with increased tolerance to multiple environmental stresses.
KeywordsAntioxidant enzyme Environmental stress Molecular breeding Potato Transgenic plant
This work was supported by grants from the Biogreen 21 Program, Rural Development Administration, Korea, from the Environmental Biotechnology National Core Research Center, KOSEF/MOST, and from the International Collaboration Project, Ministry of Science and Technology (MOST), Korea.
- Gong M, Li Y, Chen S (1998) Abscisic acid-induced thermotolerance in maize seedling is mediated by calcium and associated with antioxidant systems. J Plant Physiol 153:488–496Google Scholar
- Jang HH, Lee KO, Chi YH, Jung BG, Park JH, Lee JR, Moon JC, Yun JW, Choi YO, Kim WY, Kang JS, Cheong GW, Yun DJ, Rhee SG, Cho MJ, Lee SY (2004) Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecules chaperone function. Cell 28:625–635CrossRefGoogle Scholar
- Kim SH, Hamada T (2005) Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L.). Lam Biotech Lett 27:1841–1845Google Scholar
- Lee HS, Kim KY, You SH, Kwon SY, Kwak SS (1999) Molecular characterization and expression of a cDNA encoding copper/zinc superoxide dismutase from cultured cells of cassava (Manihot esculenta Crantz). Mol Gen Genet 262:807–814Google Scholar
- Moon H, Lee B, Choi G, Shin D, Prasad DT, Lee OS, Kwak SS, Kim DH, Nam JS, Bark J, Hong JC, Lee SY, Cho MJ, Lim CO, Yun DJ (2003) NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc Natl Acad Sci USA 100:358–363PubMedCrossRefGoogle Scholar
- Perl A, Perl-Treves R, Dalili S (1993) Enhanced oxidative stress defence in transgenic potato expressing Cu/Zn superoxide dismutase. Theor Appl Genet 85:568–576Google Scholar
- Ross H (1986) Potato breeding: problems and perspectives. J Plant Breed Suppl 13. Adv Plant Breed. Parey, Berlin and Hamburg, 132 ppGoogle Scholar
- Tang L, Kwon SY, Kwak SS, Sung CK, Lee HS (2004) Selection of transgenic potato plants expressing both CuZnSOD and APX in chloroplasts with enhanced tolerance to oxidative stress. Korean J Plant Biotechnol 31:109–113Google Scholar