Screening and expression analysis of Phytophthora infestans induced genes in potato leaves with horizontal resistance
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Horizontal resistance to late blight with quantitative and durable characteristics is a major objective for potato breeding programs. With the aim of investigating the molecular aspects of horizontal resistance, a cDNA microarray was used to identify Phytophthora infestans-induced genes from 100 expressed sequence tags (ESTs) selected from a subtractive cDNA library. Of the 100 cDNA clones represented on the array, 76 were differentially expressed in infected plants as compared with mock-inoculated control plants. Four groups of genes could be identified according to their expression patterns at three time points, 24, 48 and 72 h postinoculation (hpi). Group A appeared to be strongly induced (>10-fold) at 72 hpi. Group B demonstrated up-regulated expression patterns at all the three time points. The transcripts of group C peaked at 48 hpi, while genes of group D were up-regulated at 24 hpi and decreased slightly thereafter. Blast algorithm searches revealed that the largest set of up-regulated genes (about 35%) was assigned to the primary/secondary metabolism. Other genes with known or putative functions included disease defense or cell rescue (about 18%), transcription, signal transduction, cellular transporter/transport facilitation, development, protein synthesis/destination, as well as those playing roles in cellular organization. Furthermore, 15 genes encoding unknown function proteins were also identified. The results indicated that multiple defense mechanisms are involved in horizontal potato resistance to late blight and alteration in metabolic pathways is one of the most important defense responses.
KeywordsHorizontal resistance Late blight Pathogen-induced gene Phytophthora infestans Solanum tuberosum L.
This work was funded by the National High Technology Project of China (2202AA241181), and the National Natural Science Foundation of China (30471100), partially supported by a grant from ICGEB (The International Centre for Genetic Engineering and Biotechnology, CRP/CHN04-01).
- Avrova AO, Taleb N, Rokka VM, Heilbronn J, Campbell E, Hein I, Gilroy EM, Cardle L, Bradshaw JE, Stewart HE, Fakim YJ, Loake G, Birch PRJ (2004) Potato oxysterol binding protein and cathepsin B are rapidly up-regulated in independent defense pathways that distinguish R gene-mediated and fieldresistances to Phytophthora infestans. Mol Plant Pathol 5:45–56CrossRefPubMedGoogle Scholar
- Birch PRJ, Kamoun S (2000) Studying interaction transcriptomes: coordinated analyses of gene expression during plant–microorganism interactions. In: Wood R (ed) New technologies for life science: a trends guide. Elsevier Science, New York, pp 77–82Google Scholar
- Dellagi A, Heilbronn J, Avrova AO, Montesano M, Palva TE, Stewart HE, Toth IK, Cooke DEL, Lyon GD, Birch PRJ (2000) A potato gene encoding a WRKY-like transcription factor is induced in interactions with Erwinia carotovora subsp. atroseptica and Phytophthora infestans and is coregulated with a class I endochitinase expression. Mol Plant Microbe Interact 13:1092–1101PubMedCrossRefGoogle Scholar
- Lee S, Kim SY, Chung E, Joung YH, Pai HS, Hur CG, Choi D (2004) EST and microarray analyses of pathogen-responsive genes in hot pepper (Capsicum annuum L.) non-host resistance against soybean pustule pathogen (Xanthomonas axonopodis pv.glycines). Funct Integr Genomics 4:196–205PubMedCrossRefGoogle Scholar
- Linke C, Conrath U, Jeblick W, Betsche T, Mahn A, Duering K, Neuhaus E (2002) Inhibition of the plastidic ATP/ADP transporter protein primes potato tubers for augmented elicitation of defense responses and enhances their resistance against Erwinia carotovora. Plant Physiol 129:1607–1615PubMedCrossRefGoogle Scholar
- Mahalingam R, Gomez-Buitrago A, Eckardt N, Shah N, Guevara-Garcia A, Day P, Raina R, Fedoroff NV (2003) Characterizing the stress/defense transcriptome of Arabidopsis. Genome Biol 4(R20):1–14Google Scholar
- Pontier D, Balague C, Roby D (1999) The hypersensitive response: a programmed cell death associated with plant resistance. Mol Boil Genet 321:721–734Google Scholar
- Ronning CM, Stegalkina SS, Ascenzi RA, Bougri O, Hart AL, Utterback TR, Vanaken SE, Riedmuller SB, White JA, Cho J, Pertea GM, Lee Y, Karamycheva S, Sultana R, Tsai J, Quackenbush J, Griffiths HM, Restrepo S, Smart CD, Fry WE, Van Der Hoeven R, Tanksley S, Zhang P, Jin H, Yamamoto ML, Bake BJ, Buell CR (2003) Comparative analyses of potato expressed sequence tag libraries. Plant Physiol 131:419–429PubMedCrossRefGoogle Scholar
- Ross H (1986) Potato breeding. Problems and perspectives. Adv Plant Breed (Suppl) 13:82–86Google Scholar
- Tian ZD, Liu J, Xie CH, Song BT (2003b) Cloning of potato POTHR-1 gene and its expression in response to infection by Phytophthora infestans and other abiotic stimuli. Acta Bot Sin 45:959–965Google Scholar
- Turner JG, Ellis C, Devoto A (2002) The jasmonate signal pathway. Plant Cell 14:S153–S164Google Scholar
- Wastie RL (1991) In Phytophthora infestans: The cause of late blight of potato. In: Ingram DS, Williams PH (eds) Advances in plant pathology, vol 7. Academic Press, London, pp 193–223Google Scholar