Plant Cell Reports

, Volume 25, Issue 8, pp 792–798

Transgenic zoysiagrass (Zoysia japonica) plants obtained by Agrobacterium-mediated transformation

Genetic Transformation and Hybridization

DOI: 10.1007/s00299-006-0123-8

Cite this article as:
Ge, Y., Norton, T. & Wang, ZY. Plant Cell Rep (2006) 25: 792. doi:10.1007/s00299-006-0123-8

Abstract

Zoysiagrass (Zoysia japonica Steud.) is an important turfgrass that spreads by stolons and rhizomes. By exploring the potential of direct shoot formation from stolons, we developed a straightforward and efficient transformation protocol without callus induction and propagation. Sterilized stolon nodes were infected and co-cultivated with Agrobacterium tumefaciens harboring pCAMBIA vectors. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Both green and albino shoots were directly regenerated from the infected stolon nodes 4–5 weeks after hygromycin selection. Greenhouse-grown plants were obtained 10–12 weeks after Agrobacterium-mediated transformation. Based on the number of transgenic plants obtained and the number of stolon nodes infected, a transformation frequency of 6.8% was achieved. Stable integration of the transgenes in the plant genome was demonstrated by PCR and Southern blot hybridization analyses. Expression of the transgenes was confirmed by RT-PCR analysis and GUS staining. The new transformation system opens up new opportunities for the functional characterization of genes and promoters and the development of novel germplasm in zoysiagrass.

Keywords

Agrobacterium Genetic transformation Transgenic plant Turfgrass Zoysia japonica Zoysiagrass 

Abbreviations

hph

hygromycin phosphotransferase gene

gusA

β-glucuronidase gene

PCR

polymerase chain reaction

RT-PCR

reverse transcriptase-PCR

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Forage Improvement DivisionThe Samuel Roberts Noble FoundationArdmoreUSA

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