Plant Cell Reports

, Volume 24, Issue 8, pp 487–493 | Cite as

The ectopic expression of phenylalanine ammonia lyase with ectopic accumulation of polysaccharide-linked hydroxycinnamoyl esters in internode parenchyma of rice mutant Fukei 71

  • Kohei Mase
  • Kanna Sato
  • Yoshimi Nakano
  • Nobuyuki Nishikubo
  • Yuuri Tsuboi
  • Jinmei Zhou
  • Hidemi Kitano
  • Yoshihiro Katayama
Genetic Transformation and Hybridization
First Online:
Received:
Revised:
Accepted:

Abstract

Both polysaccharide-linked hydroxycinnamoyl esters (PHEs) and lignin are biosynthesized via the phenylpropanoid pathway. In the abnormal internode parenchyma of the rice (Oryza sativa L.) mutant Fukei 71, which has a defective recessive gene (d50), the biosynthesis of lignin and PHEs differs. . The polysaccharide-linked ferulate and p-coumarate have been shown to accumulate to high levels in the irregularly shaped and collapsed internode parenchyma cells of Fukei 71 without an accompanying overaccumulation of lignin as a result of the defective d50 gene. In the present study we demonstrated that in this abnormal parenchyma tissue of Fukei 71 the expression of phenylalanine ammonia lyase (PAL) and glutamine synthetase (GS) were ectopically induced with the ectopic accumulation of PHEs, suggesting that the d50 gene may play a role as a controlling element in the biosynthesis of PHEs during cell-wall formation in the grasses.

Keywords

Glutamine Synthetase Parenchyma Cell Phenylalanine Ammonia Lyase Phenylpropanoid Pathway Rice Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Kohei Mase
    • 1
  • Kanna Sato
    • 1
  • Yoshimi Nakano
    • 1
  • Nobuyuki Nishikubo
    • 1
    • 3
  • Yuuri Tsuboi
    • 1
  • Jinmei Zhou
    • 1
  • Hidemi Kitano
    • 2
  • Yoshihiro Katayama
    • 1
  1. 1.Graduate School of Bio-Applications and Systems EngineeringTokyo University of Agriculture and TechnologyKoganei, TokyoJapan
  2. 2.Graduate School of Bioagricultural SciencesNagoya UniversityChikusa-ku, NagoyaJapan
  3. 3.Plant Science CenterRIKENTsurumi-ku, Yokohama-shi, KanagawaJapan

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