Plant Cell Reports

, Volume 24, Issue 7, pp 392–400 | Cite as

In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants

Cell Biology and Morphogenesis
First Online:
Received:
Revised:
Accepted:

Abstract

A protocol for shoot regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. has been developed using leaf explants originating from in vitro seedlings and mature material. The explants were cultured on Murashige and Skoog medium containing various concentrations of α-naphthaleneacetic acid and thidiazuron (TDZ). Concentrations of TDZ lower than 1.0 μM promoted direct shoot regeneration, but higher concentrations promoted callus induction. Around 96–100% regeneration was obtained between 1.0 and 10 μM TDZ. The average number of shoots per explant at 1.0 μM TDZ was 8.4±4.8. Among the different explants used, the highest percentage of regeneration and shoots per explant was obtained from complete leaf explants. A significant (P≤0.05) difference in regeneration capacity was observed among the five genotypes examined. The resulting shoots were multiplied on multiplication medium, rooted and acclimatised in a greenhouse.

Keywords

Callus induction Hagenia abyssinica α-Naphthaleneacetic acid Regeneration Thidiazuron 

Abbreviations

BAP

6-Benzylaminopurine

2,4-D

2,4-Dichlorophenoxyacetic acid

IBA

Indole-3-butyric acid

MS

Murashige and Skoog medium

NAA

α-Naphthaleneacetic acid

TDZ

Thidiazuron

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Notes

Acknowledgements

This work was supported by BIO-EARN (East African Regional Programme and Research Network for Biotechnology, Biosafety and Biotechnology Policy Development) funded by SIDA/SAREC, Sweden. The authors acknowledge Mrs Annelie Ahlman for technical assistance and Dr. Salla Marttila for kind help with histological study.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Crop ScienceSwedish University of Agricultural SciencesAlnarpSweden
  2. 2.Department of Biology, Faculty of ScienceAddis Ababa UniversityAddis AbabaEthiopia

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