Plant Cell Reports

, Volume 24, Issue 4, pp 250–254 | Cite as

Abiotic metal stress enhances diosgenin yield in Dioscorea bulbifera L. cultures

Biotic and Abiotic Stress


Lower concentrations of CuSO4 (25–75 μM) in the MS medium supplemented with 0.1 mg l−1 IAA+5.0 mg l−1 Kn+500 mg l−1 CH+10 mg l−1 Cyst hyd enhanced the growth of regenerants of Dioscorea bulbifera L. CuSO4 (75 μM) induced an appreciable diosgenin yield in the regenerants compared to those obtained on media without Cu. The presence of Cu thus seems to stimulate diosgenin production. The regenerants also differentiated bulbils on lower concentrations of Cu. At CuSO4 (100 μM), however, cultures showed poor growth as well as a low diosgenin yield. Increased proline and protein contents were recorded in cultures grown on Cu-enriched media.


Copper sulphate Diosgenin Regeneration Proline Protein 



Casein hydrolysate

Cyst hyd:

Cysteine hydrochloride


Dry weight


Fresh weight


Indole-3-acetic acid




Murashige and Skoog medium


Trichloroacetic acid


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of BiotechnologyHamdard UniversityNew DelhiIndia

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