Plant Cell Reports

, Volume 24, Issue 4, pp 250–254 | Cite as

Abiotic metal stress enhances diosgenin yield in Dioscorea bulbifera L. cultures

Biotic and Abiotic Stress

Abstract

Lower concentrations of CuSO4 (25–75 μM) in the MS medium supplemented with 0.1 mg l−1 IAA+5.0 mg l−1 Kn+500 mg l−1 CH+10 mg l−1 Cyst hyd enhanced the growth of regenerants of Dioscorea bulbifera L. CuSO4 (75 μM) induced an appreciable diosgenin yield in the regenerants compared to those obtained on media without Cu. The presence of Cu thus seems to stimulate diosgenin production. The regenerants also differentiated bulbils on lower concentrations of Cu. At CuSO4 (100 μM), however, cultures showed poor growth as well as a low diosgenin yield. Increased proline and protein contents were recorded in cultures grown on Cu-enriched media.

Keywords

Copper sulphate Diosgenin Regeneration Proline Protein 

Abbreviations

CH:

Casein hydrolysate

Cyst hyd:

Cysteine hydrochloride

dw:

Dry weight

fw:

Fresh weight

IAA:

Indole-3-acetic acid

Kn:

Kinetin

MS:

Murashige and Skoog medium

TCA:

Trichloroacetic acid

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of BiotechnologyHamdard UniversityNew DelhiIndia

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