Plant Cell Reports

, Volume 25, Issue 6, pp 607–612 | Cite as

Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures

Physiology and Biochemistry

Abstract

Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced with MJ (100 μM) on day 6 and fed loganin (0.30 mM), tryptamine (0.15 mM), loganin plus tryptamine, or geraniol (0.1–1.0 mM) on day 7. While MJ increased ajmalicine production by 3-fold, induced cultures were still limited by terpenoid precursors. However, both induced and non-induced cultures became tryptamine-limited with excess loganin. Geraniol feeding also increased ajmalicine production in non-induced cultures. But MJ appeared to increase geraniol availability in induced cultures, due presumably to the increased expression of Dxs with MJ addition.

Keywords

Ajmalicine Catharanthus roseus Loganin Methyl jasmonate Precursor feeding Tryptamine 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Chemical Engineering Department, 342 Snell Engineering CenterNortheastern UniversityBostonUSA

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