Plant Cell Reports

, Volume 25, Issue 4, pp 349–358

Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants

PHYSIOLOGY AND BIOCHEMISTRY

DOI: 10.1007/s00299-005-0093-2

Cite this article as:
Cho, E.K. & Hong, C.B. Plant Cell Rep (2006) 25: 349. doi:10.1007/s00299-005-0093-2
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Abstract

HSP70, a heat shock protein, is a molecular chaperone responsive to various environmental stresses. Here, NtHSP70-1 (AY372069) was a drought-/ABA-inducible gene. We monitored the expression of CaERD15 (early responsive to dehydration, DQ267932) with exposing plants to progressive drought stress. Its activity was used as an indicator of water-deficit conditions. To analyze the protective role of HSP70, we obtained transgenic tobacco plants that constitutively expressed elevated levels of the tobacco HSP70, NtHSP70-1, as well as transgenic plants containing either the vector alone or else having NtHSP70-1 in the antisense orientation. Plants with enhanced levels of NtHSP70-1 in their transgenic sense lines exhibited tolerance to water stress. Under progressive drought, the amount of leaf NtHSP70-1 was correlated with maintenance of optimum water content, with contents being higher in the leaves of dehydrated transgenic sense plants than in those of either the control (vector-only) or the transgenic antisense plants. Moreover, the expression of CaERD15 was considerably reduced in tobacco plants that over-expressed NtHSP70-1. These results suggest that elevated levels of NtHSP70-1 can confer drought-stress tolerance.

Keywords

CaERD15 Drought NtHSP70-1 Tobacco Transgenic plants ABA 

Abbreviations

NtHSP70-1

Nicotiana tabacum Heat Shock Protein 70-1

CaERD15

Capsicum annuum early response to dehydration gene 15

ABA

abscisic acid

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Institute of Molecular Biology and Genetics and School of Biological SciencesSeoul National UniversitySeoulKorea

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