Plant Cell Reports

, Volume 25, Issue 4, pp 349–358 | Cite as

Over-expression of tobacco NtHSP70-1 contributes to drought-stress tolerance in plants

PHYSIOLOGY AND BIOCHEMISTRY

Abstract

HSP70, a heat shock protein, is a molecular chaperone responsive to various environmental stresses. Here, NtHSP70-1 (AY372069) was a drought-/ABA-inducible gene. We monitored the expression of CaERD15 (early responsive to dehydration, DQ267932) with exposing plants to progressive drought stress. Its activity was used as an indicator of water-deficit conditions. To analyze the protective role of HSP70, we obtained transgenic tobacco plants that constitutively expressed elevated levels of the tobacco HSP70, NtHSP70-1, as well as transgenic plants containing either the vector alone or else having NtHSP70-1 in the antisense orientation. Plants with enhanced levels of NtHSP70-1 in their transgenic sense lines exhibited tolerance to water stress. Under progressive drought, the amount of leaf NtHSP70-1 was correlated with maintenance of optimum water content, with contents being higher in the leaves of dehydrated transgenic sense plants than in those of either the control (vector-only) or the transgenic antisense plants. Moreover, the expression of CaERD15 was considerably reduced in tobacco plants that over-expressed NtHSP70-1. These results suggest that elevated levels of NtHSP70-1 can confer drought-stress tolerance.

Keywords

CaERD15 Drought NtHSP70-1 Tobacco Transgenic plants ABA 

Abbreviations

NtHSP70-1

Nicotiana tabacum Heat Shock Protein 70-1

CaERD15

Capsicum annuum early response to dehydration gene 15

ABA

abscisic acid

References

  1. Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78PubMedCrossRefGoogle Scholar
  2. Albrecht M, Lengauer T (2004) Survey on the PABC recognition motif PAM2. Biochem Biophys Res Commun 316:129–138PubMedCrossRefGoogle Scholar
  3. Alvim FC, Carolino SMB, Cascardo JCM, Nunes CC, Martinez CA, Otoni WC, Fontes EPB (2001) Enhanced accumulation of BiP in transgenic plants confers tolerance to water stress. Plant Physiol 126:1042–1054PubMedCrossRefGoogle Scholar
  4. An G, Ebert PR, Mitre A, Ha SB (1988) Binary vectors. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual, Kluwer, Dordrecht, pp 1–19Google Scholar
  5. Bartels D, Schneider K, Terstappen G, Piatkowski D, Salamini F (1990) Molecular cloning of abscisic acid-modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181:27–34CrossRefGoogle Scholar
  6. Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111PubMedCrossRefGoogle Scholar
  7. Bowler C, Fluhr R (2000) The molecular basis of cross tolerance. Trends Plant Sci 5:241–246PubMedCrossRefGoogle Scholar
  8. Boyer EA (1993) Plant productivity and environment. Science 218:443–448CrossRefGoogle Scholar
  9. Bray EA (1997) Plant responses to water deficit. Trends Plant Sci 2:45–54CrossRefGoogle Scholar
  10. Cellier F, Conéjéro G, Breitler JC, Casse F (1998) Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower. Plant Physiol 116:319–328PubMedCrossRefGoogle Scholar
  11. Chandler PM, Robertson M (1994) Gene expression regulated by abscisic acid and its relation to stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 45:113–141CrossRefGoogle Scholar
  12. Cho EK, Hong CB (2004) Molecular cloning and expression pattern analyses of heat shock protein 70 genes from Nicotiana tabacum. J Plant Biol 47:149–159CrossRefGoogle Scholar
  13. Cho YB, Jeon HJ, Hong CB (2003) Cloning and functional annotation of rare mRNA species from drought-stressed hot pepper (Capsicum annuum). J Plant Biol 46:83–89CrossRefGoogle Scholar
  14. Dat J, Vandenbeele S, Vranova E, van Montagu M, Inze D, van Breusegm F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795PubMedCrossRefGoogle Scholar
  15. Dix DJ (1997) Hsp70 expression during gametogenesis. Cell Stress Chap 2:73–77CrossRefGoogle Scholar
  16. Doczi R, Kondrak M, Kovacs G, Beczner F, Banfalvi Z (2005) Conservation of the drought-inducible DS2 genes and divergences from their ASR paralogues in solanaceous species. Plant Physiol Biochem 43:269–276PubMedCrossRefGoogle Scholar
  17. Feder ME, Hofmann GE (1999) Heat-shock protein, molecular chaperones, and the stress response: Evolutionary and ecological physiology. Annu Rev Physiol 61:243–282PubMedCrossRefGoogle Scholar
  18. Giraudat J, Parcy F, Bertauche N, Gosti F, Leung J, Morris PC, Bouvierdurand M, Vartanian N (1994) Current advances in abscisic acid action and signaling. Plant Mol Biol 26:1557–1577PubMedCrossRefGoogle Scholar
  19. Hammond C, Helenius A (1995) Quality control in the secretory pathway. Curr Opin Cell Biol 7:523–529PubMedCrossRefGoogle Scholar
  20. Hoekstra FA, Golovina EA, Buitink J (2001) Mechanisms of plant desiccation tolerance. Trends Plant Sci 6:431–438PubMedCrossRefGoogle Scholar
  21. Horsch RB, Fry JE, Hoffman NC, Eicholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transforming gene into plants. Science 227:1229–1231CrossRefGoogle Scholar
  22. Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol 47:377–403PubMedCrossRefGoogle Scholar
  23. Kahvejian A, Roy G, Sonenberg N (2001) The mRNA closed loop model: the function of PABP and PABP-interacting proteins in mRNA translation. Cold Spring Harbor Symp Quant Biol 66:293–300PubMedCrossRefGoogle Scholar
  24. Kariola T, Helenius E, Brader G, Li J, Heino P, Palva ET (2005) ERD15 modulates abiotic stress responses in Arabidopsis. The 5th Workshop in the Nordic Arabidopsis Network, DenmarkGoogle Scholar
  25. Kim HS, Lee JH, Kim JJ, Kim CH, Jun SS, Hong YN (2005) Molecular and functional characterization of CaLEA6, the gene for a hydrophobic LEA protein from Capsicum annuum. Gene 344:115–123PubMedCrossRefGoogle Scholar
  26. Kiyosue T, Abe H, Yamaguchi-Shinozaki K, Shinozaki K (1998) ERD6, a cDNA clone for an early dehydration-induced gene of Arabidopsis, encodes a putative sugar transporter. Biochim Biophys Acta 1370:187–191PubMedCrossRefGoogle Scholar
  27. Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K (1994a) ERD15, a cDNA for a dehydration-induced gene from Arabidopsis thaliana. Plant Physiol 106:1707–1712PubMedCrossRefGoogle Scholar
  28. Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K (1994b) Cloning of cDNA for genes that are early-response to dehydration stress (ERDs) in Arabidopsis thaliana L.: Identification of three ERDs as HSP cognate genes. Plant Mol Biol 25:791–798PubMedCrossRefGoogle Scholar
  29. Krebs RA, Feder ME (1997) Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae. Cell Stress Chap 2:60–71CrossRefGoogle Scholar
  30. Lang V, Welin B, Sundberg B, Palva ET (1994) Alterations in water status, endogenous abscisic acid content, and expression of rab18 gene during the development of freezing tolerance in Arabidopsis thaliana. Plant Physiol 104:1341–1349PubMedGoogle Scholar
  31. Lee GJ, Vierling E (2000) A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein. Plant Physiol 122:189–198PubMedCrossRefGoogle Scholar
  32. Lee JH, Schoffl F (1996) An Hsp70 antisense gene affects the expression of HSP70/HSC70, the regulation of HSF, and the acquisition of thermotolerance in transgenic Arabidopsis thaliana. Mol Gen Genet 252:11–19PubMedCrossRefGoogle Scholar
  33. Luft JC, Dix DJ (1999) HSP70 expression and function during embryogenesis. Cell Stress Chap 4:162–170CrossRefGoogle Scholar
  34. Mittler R (2002) Oxidative stress, antioxidants, and stress tolerance. Trends Plant Sci 7:405–410PubMedCrossRefGoogle Scholar
  35. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15:473–497CrossRefGoogle Scholar
  36. Oono Y, Seki M, Nanjo T, Narusaka M, Fujita M, Satoh R, Satou M, Sakurai T, Ishida J, Akiyama K, Iida K, Maruyama K, Satoh S, Yamaguchi-Shinozaki K, Shinozaki K (2003) Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca. 7000 full-length cDNA microarray. Plant J 34:868–887PubMedCrossRefGoogle Scholar
  37. Richard S, Morency MJ, Drevet C, Jouanin L, Séguin A (2000) Isolation and characterization of a dehydrin gene from white spruce induced upon wounding, drought and cold stresses. Plant Mol Biol 43:1–10PubMedCrossRefGoogle Scholar
  38. Rizhsky L, Liang H, Mittler R (2002) The combined effect of drought stress and heat shock on gene expression in tobacco. Plant Physiol 130:1143–1151PubMedCrossRefGoogle Scholar
  39. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  40. Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J 31:279–292.PubMedCrossRefGoogle Scholar
  41. Shinozaki K, Yamaguchi-Shinozaki K (1996) Molecular responses to drought and cold stress. Curr Opin Biotechnol 7:161–167PubMedCrossRefGoogle Scholar
  42. Shinozaki K, Yamaguchi-Shinozaki K (1997) Gene expression and signal transduction in water-stress response. Plant Physiol 115:327–334PubMedCrossRefGoogle Scholar
  43. Suh MC, Hong CB, Kim SS, Sim WS (1994) Transgenic tobacco plants with Bacillus thuringiensis delta-toxin gene resistant to Korean born tobacco budworms. Mol Cells 4:211–219Google Scholar
  44. Takahashi S, Seki M, Ishida J, Satou M, Sakurai T, Narusaka M, Kamiya A, Nakajima M, Enju A, Akiyama K, Yamaguchi-Shinozaki K, Shinozaki K (2004) Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray. Plant Mol Biol 56:29–55PubMedCrossRefGoogle Scholar
  45. Tanford C (1978) The hydrophobic effect and the organization of living matter. Science 200:1012–1018PubMedCrossRefGoogle Scholar
  46. Vierling E (1991) The roles of heat shock proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 42:579–620CrossRefGoogle Scholar
  47. Wang X, Grumet R (2003) Identification and characterization of proteins that interact with the carboxy terminus of poly(A)-binding protein and inhibit translation in vitro. Plant Mol Biol 54:85–98CrossRefGoogle Scholar
  48. Xiong L, Zhu JK (2002) Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell Environ 25:131–139PubMedCrossRefGoogle Scholar
  49. Yoshioka R, Soga K, Wakabayashi K, Takeba G, Hoson T (2003) Hypergravity-induced changes in gene expression in Arabidopsis hypocotyls. Adv Space Res 31:2187–2193PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Institute of Molecular Biology and Genetics and School of Biological SciencesSeoul National UniversitySeoulKorea

Personalised recommendations