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Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism

  • Cell Biology and Morphogenesis
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Abstract

The effect of donor plants annual cycle and anther/spike position on the production of microspore-derived plants and albinism were studied. We used the winter cv. Igri and the spring cv. Cork, known to respond similarly in anther culture but to produce 78% and 2% of green plants, respectively. In both cvs. the number of microspore-derived plants was significantly higher when the anthers were collected from January to July than from August to December. However, during this period the proportion of albino plants was not altered. Conversely, the anther response decreased from 76.6 to 31.5% in Igri and from 58.8 to 32.0% in Cork when the donor spike originates from the main shoot or the fourth tiller. Significantly, anthers collected from spike of the second tiller enabled us to drastically increase the proportion of regenerated green plantlets, by 16% in Igri and 1800% in Cork.

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Abbreviations

BBCH:

Biologische Bundesamstalt für Land und Forstwirtschaft Bundessortenamt und CHemische Industrie

cv.:

cultivar

cvs.:

cultivars

MS:

main shoot

SE:

standard error

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Acknowledgement

The authors thank Dr Steve Millan for the English correction of the manuscript.

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Authors and Affiliations

  1. Université de Reims Champagne-Ardenne, UFR Sciences, Stress Défenses et Reproduction des Plantes, 51687, Reims Cedex 2, France

    C. Jacquard, A. M. Hamalian & C. Clément

  2. Voke branch of Lithuania Institute of Agriculture, Zalioji a. 2, Traku Voke, LT-02232, Vilnius, Lithuania

    R. Asakaviciute

  3. Université de Picardie Jules Verne, Androgenèse et Biotechnologies, 33, rue Saint-Leu, 80039, Amiens, France

    R. S. Sangwan

  4. Florimond Desprez, Laboratoire Biotechnologies, BP 41, 59242, Cappelle en Pévèle, France

    P. Devaux

Authors
  1. C. Jacquard
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  2. R. Asakaviciute
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  3. A. M. Hamalian
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  4. R. S. Sangwan
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  5. P. Devaux
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  6. C. Clément
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Corresponding author

Correspondence to C. Clément.

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Communicated by H. Lörz

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Jacquard, C., Asakaviciute, R., Hamalian, A.M. et al. Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism. Plant Cell Rep 25, 375–381 (2006). https://doi.org/10.1007/s00299-005-0070-9

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  • DOI: https://doi.org/10.1007/s00299-005-0070-9

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