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Plant Cell Reports

, Volume 25, Issue 4, pp 281–288 | Cite as

TDZ, auxin and genotype effects on leaf organogenesis in Fragaria

  • L. Landi
  • B. Mezzetti
Cell Biology and Morphogenesis

Abstract

The different types of organogenic (roots and adventitious shoots) and callus formation responses of leaves from 30-day-old proliferating shoots of different Fragaria spp. genotypes were studied in response to MS medium supplemented with 4.54 μM 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron; TDZ) alone and in combination with 0.98 μM indole-3-butyric acid (IBA), 0.84 μM3-benzo[b]selenienyl acetic acid (BSAA) or 0.90 μM2,4-dichlorophenoxy acetic acid (2,4-D). The study included: nine octoploid Fragaria x ananassa cultivars and breeding selections; two octoploid breeding selections from F. virginiana glauca inter-species crosses; two diploid F. vesca cultivars; and one diploid clone of F. nubicola Lindl. TDZ plus IBA promoted the highest shoot regeneration efficiencies from leaves of nearly all of the genotypes, while the TDZ/BSAA and TDZ/2,4-D combinations promoted high regeneration efficiencies for only some of the genotypes (Alpina W.O., Sveva, AN 91.371.53, Onda, Paros and FO93.143.5). For the more efficient regenerating genotypes, IBA induced the highest frequency of regenerating leaves, while BSAA induced the highest number of regenerated shoots from leaves and more callus production for most of the genotypes.

Keywords

Strawberry Leaf tissue TDZ BSAA Shoot regeneration 

Abbreviations

2,4-D

2,4-Dichlorophenoxy acetic acid

BA

6-Benzylaminopurine

BSAA

3-Benzo[b] selenienyl acetic acid

IBA

Indole-3-butyric acid

IAA

Indole-3-acetic acid

MS medium

Murashige and Skoog (1962) medium

PGR

Plant growth regulator

TDZ

(thidiazuron)1-Phenyl-3-(1,2,3-thiadiazol-5-yl) urea

Notes

Acknowledgements

This research was financed by the MIUR (Italian Ministry of University and Research)—FIRB project N. RBAU01JTHS (www.bioinformatics.unito/bioinformatica/Spena) and by the PRIN project 2002078882_002.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Dipartimento di Scienze Ambientali e delle Produzioni VegetaliMarche Polytechnic UniversityVia Brecce BiancheItaly

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