Plant Cell Reports

, Volume 25, Issue 3, pp 231–240

Characterization of an anther- and tapetum-specific gene and its highly specific promoter isolated from tomato

Physiology and Biochemistry

DOI: 10.1007/s00299-005-0056-7

Cite this article as:
Xu, S.X., Liu, G.S. & Chen, R.D. Plant Cell Rep (2006) 25: 231. doi:10.1007/s00299-005-0056-7

Abstract

A full-length genomic clone of 2,233 bp long containing an anther- and tapetum-specific gene TomA108 was isolated and characterized from tomato. The gene was present in one copy per haploid genome. The isolated clone contained 5′ and 3′ untranslated regions of 810 and 170 nucleotides, respectively and a single intron with highly repetitive sequences. The cDNA encoded the protein with an apparent mass of 10.6 kDa and a pI (isoelectric point) of 5.3. It was cysteine-rich and had an N-terminal hydrophobic domain with characteristics of a secretory signal. Amino acid sequence comparisons demonstrated that the protein was closely related to a family of cereal seed storage proteins and protease inhibitors. The fusion of β-glucuronidase to the TomA108 promoter demonstrated that the promoter was highly active from early-meiosis to free microspores production in tapetum of tobacco. This strong and highly specific promoter can be potentially used to generate male sterility for efficient production of plant hybrids.

Keywords

Anther Cysteine-rich Promoter Tapetum 

Abbreviations

GUS

β-Glucuronidase

EDTA

Ethylene diamine tetraacetie acid

MADS

MCM1, AGAMOUS, DEFICIENS, and SRF

CarG

(CC(A/T)6GG)

GA

Gibbrerllin

ABA

Abscisic acid

SA

Salicylic acid

SDS

Sodium dodecyl sulfate

IPTG

Isopropyl-1-thio-β-d-alactoside

PAGE

Polyacrylamide gel electrophoresis

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Key Laboratory of Photosynthesis and Environmental Molecular PhysiologyInstitute of Botany, Chinese Academy of SciencesBeijingChina
  2. 2.Department of BiochemistryUniversity of SaskatchewanSaskatoonCanada

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