Plant Cell Reports

, 25:124 | Cite as

Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola

  • Ri-He Peng
  • Quan-Hong Yao
  • Ai-Sheng Xiong
  • Zong-Ming Cheng
  • Yi Li
Genetic Transformation and Hybridization

Abstract

Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobacterium-mediated transformation. Phytase expression in transgenic plant was enhanced with a synthetic phytase gene according to the Brassica codon usage and an endoplasmic reticulum (ER) retention signal KDEL that confers an ER accumulation of the recombinant phytase. Secretion of the phytase to the extracellular fluid was also established by the use of the tobacco PR-S signal peptide. Phytase accumulation in mature seed accounted for 2.6% of the total soluble proteins. The enzyme can be glycosylated in the seeds of transgenic plants and retain a high stability during storage. These results suggest a commercial feasibility of producing a stable recombinant phytase in canola at a high level for animal feed supplement and for reducing phosphorus eutrophication problems.

Keywords

Agrobacterium-mediated transformation Aspergillus phytase Brassica napus Codon modification Endoplasmic reticulum retention signal 

Abbreviations

GUS

β-glucuronidase

MPHY2

modified phytase gene

PR-S

pathogen-related protein S

SAR

scaffold attachment regions

Notes

Acknowledgements

This research was supported by the Science Commission of Shanghai, project number 993913002. We thank Lori Osburn for proof-reading the manuscript.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Ri-He Peng
    • 1
  • Quan-Hong Yao
    • 1
  • Ai-Sheng Xiong
    • 1
  • Zong-Ming Cheng
    • 2
  • Yi Li
    • 3
  1. 1.Shanghai Key Laboratory of Agricultural Genetics and BreedingAgro-Biotechnology Research Center, Shanghai Academy of Agricultural SciencesShanghaiPeople's Republic China
  2. 2.Department of Plant SciencesUniversity of TennesseeKnoxvilleUSA
  3. 3.Department of Plant ScienceUniversity of ConnecticutStorrsUSA

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