Plant Cell Reports

, Volume 24, Issue 2, pp 103–111 | Cite as

Insect-resistant transgenic Pinus radiata

  • Lynette J. Grace
  • Julia A. Charity
  • Belinda Gresham
  • Nod Kay
  • Christian Walter
Genetic Transformation and Hybridization

Abstract

Transgenic radiata pine (Pinus radiata D. Don) plants containing a Bacillus thuringiensis (Bt) toxin gene, crylAc, were produced by means of biolistic transformation of embryogenic tissue. Using the selectable marker gene nptII and corresponding geneticin selection, 20 independent transgenic lines from five genotypes were established. Over 200 plants regenerated from ten transgenic lines were successfully transferred to soil. The integration and expression of the introduced genes in transgenic tissue and/or plants were confirmed by PCR, Southern hybridisation and neomycin phosphotransferase II (NPTII) and Bt ELISA assays. Bioassays with larvae of the painted apple moth, Teia anartoides, demonstrated that transgenic plants displayed variable levels of resistance to insect damage, with one transgenic line being highly resistant to feeding damage.

Keywords

Biolistic transformation Embryogenic tissue cry1Ac Conifers 

Abbreviations

ELISA

Enzyme-linked immunosorbent assay

nptII/NPTII

Neomycin phosphotransferase gene or protein

uidA/GUS

β-Glucuronidase gene or protein

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Lynette J. Grace
    • 1
  • Julia A. Charity
    • 1
  • Belinda Gresham
    • 1
  • Nod Kay
    • 1
  • Christian Walter
    • 1
  1. 1.New Zealand Forest Research Institute LtdRotoruaNew Zealand

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