Plant Cell Reports

, Volume 24, Issue 2, pp 69–78

Prevention of flower development in birch and other plants using a BpFULL1::BARNASE construct

  • M. Lännenpää
  • M. Hassinen
  • A. Ranki
  • M. Hölttä-Vuori
  • J. Lemmetyinen
  • K. Keinonen
  • T. Sopanen
Genetic Transformation and Hybridization
  • 194 Downloads

Abstract

The prevention of flower formation is important for avoiding the spread of transgenes from genetically modified plants into wild populations. Moreover, the resources not expended for the generation of flowers and fruits might be allocated to increased vegetative growth. We have been developing methods for preventing flower formation in silver birch (Betula pendula), a tree species of considerable economical importance in the boreal region. Here we study the suitability of the promoter of BpFRUITFULL-LIKE1 (BpFULL1, formerly BpMADS5) for tissue-specific ablation of inflorescences in Arabidopsis, tobacco and birch. With all these species, the development of inflorescences was successfully prevented. The results show that the BpFULL1::BARNASE construct has potential biotechnological applications in different plant species.

Keywords

Ablation BARNASE Betula pendula Flower development FRUITFULL 

References

  1. Atkinson MD (1992) Biological flora of the British isles. Betula pendula Roth (B. verrucosa Ehrh.) and B. pubescens Ehrh. J Ecol 80:837–870Google Scholar
  2. Beals TP, Goldberg RB (1997) A novel cell ablation strategy blocks tobacco anther dehiscence. Plant Cell 9:1527–1545CrossRefPubMedGoogle Scholar
  3. Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci Ser D 316:1194–1199Google Scholar
  4. Burgess DG, Ralston EJ, Hanson WG, Heckert M, Ho M, Jenq T, Palys JM, Tang K, Gutterson N (2002) A novel, two-component system for cell lethality and its use in engineering nuclear male-sterility in plants. Plant J 31:113–125CrossRefGoogle Scholar
  5. Elo A, Lemmetyinen J, Turunen M-L, Tikka L, Sopanen T (2001) Three MADS-box genes similar to APETALA1 and FRUITFULL from silver birch (Betula pendula). Physiol Plant 112:95–103PubMedGoogle Scholar
  6. Ferrandiz C, Gu Q, Martienssen R, Yanofsky MF (2000a) Redundant regulation of meristem identity and plant architecture by FRUITFULL, APETALA1 and CAULIFLOWER. Development 127:725–734Google Scholar
  7. Ferrandiz C, Liljegren SJ, Yanofsky MF (2000b) Negative regulation of the SHATTERPROOF genes by FRUITFULL during arabidopsis fruit development. Science 289:436–438CrossRefGoogle Scholar
  8. Goldman MHS, Goldberg RB, Mariani C (1994) Female sterile tobacco plants are produced by stigma-specific cell ablation. EMBO J 13:2976–2984Google Scholar
  9. Gu Q, Ferrandiz C, Yanofsky MF, Martienssen R (1998) The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development. Development 125:1509–1517Google Scholar
  10. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231Google Scholar
  11. Järvinen P, Lemmetyinen J, Savolainen O, Sopanen T (2003) DNA sequence variation in BpMADS2 gene in two populations of Betula pendula. Mol Ecol 12:369–384CrossRefGoogle Scholar
  12. Keinonen K (1999) Towards genetic manipulation of silver birch (Betula pendula) and Scots pine (Pinus sylvestris). PhD thesis, University of Joensuu, FinlandGoogle Scholar
  13. Lemmetyinen J, Keinonen-Mettälä K, Lännenpää M, von Weissenberg K, Sopanen T (1998) Activity of the CaMV 35S promoter in various parts of transgenic early flowering birch clones. Plant Cell Rep 18:243–248CrossRefGoogle Scholar
  14. Lemmetyinen J, Pennanen T, Lännenpää M, Sopanen T (2001) Prevention of flower formation in dicotyledons. Mol Breed 7:341–350CrossRefGoogle Scholar
  15. Lemmetyinen J, Hassinen M, Elo A, Porali I, Keinonen K, Mäkelä H, Sopanen T (2004a) Functional characterisation of SEPALLATA3 and AGAMOUS orthologues in silver birch. Physiol Plant 121:149–162CrossRefGoogle Scholar
  16. Lemmetyinen J, Keinonen K, Sopanen T (2004b) Prevention of the flowering of a tree, silver birch. Mol Breed 13:243–249CrossRefGoogle Scholar
  17. Longman KA, Wareing PF (1959) Early induction of flowering in birch seedlings. Nature 184:2037–2038Google Scholar
  18. Lännenpää M, Jänönen I, Hölttä-Vuori M, Gardemeister M, Porali I, Sopanen T (2004) A new SBP-box gene BpSPL1 in silver birch (Betula pendula). Physiol Plant 120:491–500CrossRefGoogle Scholar
  19. Macdonald AD, Mothersill DH (1986) Shoot development in Betula papyrifera. VI. Development of the reproductive structures. Can J Bot 65:466–475Google Scholar
  20. Mandel MA, Yanofsky MF (1995) The arabidopsis AGL8 MADS box gene is expressed in inflorescence meristems and is negatively regulated by APETALA1. Plant Cell 7:1763–1771CrossRefGoogle Scholar
  21. Mariani C, De Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347:737–741CrossRefGoogle Scholar
  22. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497 Google Scholar
  23. Pappinen A, Degefu Y, Syrjälä L, Keinonen K, Weissenberg K (2002) Transgenic silver birch (Betula pendula) expressing sugarbeet chitinase 4 shows enhanced resistance to Pyrenopeziza betulicola. Plant Cell Rep 20:1046–1051CrossRefGoogle Scholar
  24. Paul W, Hodge R, Smartt S, Draper J, Scott R (1992) The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene. Plant Mol Biol 19:611–622CrossRefGoogle Scholar
  25. Viherä-Aarnio A, Ryynänen L (1994) Seed production of micropropagated plants, grafts and seedlings of birch in a seed orchard. Silva Fenn 28:257–263Google Scholar
  26. Vivian-Smith A, Luo M, Chaudhury A, Koltunow A (2001) Fruit development is actively restricted in the absence of fertilization in Arabidopsis. Development 128:2321–2331Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • M. Lännenpää
    • 1
  • M. Hassinen
    • 1
  • A. Ranki
    • 1
  • M. Hölttä-Vuori
    • 1
  • J. Lemmetyinen
    • 1
  • K. Keinonen
    • 1
  • T. Sopanen
    • 1
  1. 1.Department of BiologyUniversity of JoensuuJoensuuFinland

Personalised recommendations