Plant Cell Reports

, Volume 23, Issue 12, pp 780–789 | Cite as

A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques

  • S. Travella
  • S. M. Ross
  • J. Harden
  • C. Everett
  • J. W. Snape
  • W. A. Harwood
Genetic Transformation and Hybridization


Two barley transformation systems, Agrobacterium-mediated and particle bombardment, were compared in terms of transformation efficiency, transgene copy number, expression, inheritance and physical structure of the transgenic loci using fluorescence in situ hybridisation (FISH). The efficiency of Agrobacterium-mediated transformation was double that obtained with particle bombardment. While 100% of the Agrobacterium-derived lines integrated between one and three copies of the transgene, 60% of the transgenic lines derived by particle bombardment integrated more than eight copies of the transgene. In most of the Agrobacterium-derived lines, the integrated T-DNA was stable and inherited as a simple Mendelian trait. Transgene silencing was frequently observed in the T1 populations of the bombardment-derived lines. The FISH technique was able to reveal additional details of the transgene integration site. For the efficient production of transgenic barley plants, with stable transgene expression and reduced silencing, the Agrobacterium-mediated method appears to offer significant advantages over particle bombardment.


Barley Agrobacterium Biolistic Transgene analysis FISH 



We gratefully acknowledge Dr. H. Salvo-Garrido for helpful discussions. Funding for this research was provided by the European Commission (4th program FAIR), the Biotechnology and Biological Sciences Research Council and the Department of the Environment, Food and Rural Affairs (DEFRA)


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • S. Travella
    • 1
    • 2
  • S. M. Ross
    • 1
  • J. Harden
    • 1
  • C. Everett
    • 1
  • J. W. Snape
    • 1
  • W. A. Harwood
    • 1
  1. 1.John Innes Centre NorwichUK
  2. 2.Present address: Institute of Plant BiologyUniversity of ZürichZürichSwitzerland

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