Plant Cell Reports

, Volume 23, Issue 12, pp 834–841 | Cite as

Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant

  • Marta Libik
  • Robert Konieczny
  • Beata Pater
  • Ireneusz Ślesak
  • Zbigniew Miszalski
Physiology and Biochemistry


Callus was obtained from hypocotyls of Mesembryanthemum crystallinum seedlings cultured on two types of medium—germination medium (GM) and callus induction medium (CIM). Following subculture on shoot induction medium SIM1, the callus formed on CIM medium regenerated roots or somatic embryos, while that obtained on GM medium was non-regenerative. The activities of CuZn-superoxidase dismutase (SOD) were comparable in all calli, but the activities of FeSOD and MnSOD varied according to the activity of photosystem II and the regenerative potential of the tissues. Catalase (CAT) activity was related to H2O2 concentration and affected by both the culture conditions and the morphogenic potential of the calli. The possible role of CAT, SODs and H2O2 in the regeneration of M. crystallinum from callus is discussed.


Catalase Hydrogen peroxide Mesembryanthemum crystallinum Plant regeneration Superoxide dismutase 





Bovine serum albumin


Crassulacean acid metabolism


Catalase [EC]




2,4-Dichlorophenoxyacetic acid


Ethylenediaminetetraacetic acid


Maximum chlorophyll a fluorescence


Minimum chlorophyll a fluorescence


Maximum photochemical efficiency of photosystem II (Fv, the difference between Fm and F0)


α-Naphthaleneacetic acid


Nitro blue tetrazolium salt


Polyacrylamide gel electrophoresis


Polyethylene glycol


Relative humidity


Reactive oxygen species


Superoxide dismutase [EC]







This study was partly supported by Polish KBN grants 6P04C 00320, 6P04F 03420, 3P04C06423 and EU grant QoL-2001-Integr to the Institute of Plant Physiology Polish Academy of Science and Deutsche Forschungsanstalt für Luft-und Raumfahrt (DLR). The Deutsche Akademische Austauschdienst (DAAD) provided the growth chamber.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Marta Libik
    • 1
  • Robert Konieczny
    • 2
  • Beata Pater
    • 1
  • Ireneusz Ślesak
    • 1
  • Zbigniew Miszalski
    • 1
    • 3
  1. 1.Institute of Plant PhysiologyPolish Academy of SciencesCracowPoland
  2. 2.Department of Plant Cytology and EmbryologyJagiellonian UniversityCracowPoland
  3. 3.Institute of BiologyPedagogical AcademyCracowPoland

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