Plant Cell Reports

, Volume 23, Issue 8, pp 557–566

Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites

  • Dong-Woog Choi
  • JongDuk Jung
  • Young Im Ha
  • Hyun-Woo Park
  • Dong Su In
  • Hwa-Jee Chung
  • Jang Ryol Liu
Genetics and Genomics

DOI: 10.1007/s00299-004-0845-4

Cite this article as:
Choi, DW., Jung, J., Ha, Y.I. et al. Plant Cell Rep (2005) 23: 557. doi:10.1007/s00299-004-0845-4

Abstract

Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment.

Keywords

Expression sequence tags Methyl jasmonate Secondary metabolism Ginsenoside biosynthesis Panax ginseng 

Abbreviations

β-AS

β-Amyrin synthase

DS

Dammarendiol synthase

ESTs

Expression sequence tags

LS

Lupeol synthase

MeJA

Methyl jasmonic acid

OSC

Oxidosqualene cyclase

SE

Squalene epoxidase

SS

Squalene synthase

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Dong-Woog Choi
    • 1
    • 3
  • JongDuk Jung
    • 1
  • Young Im Ha
    • 1
  • Hyun-Woo Park
    • 1
  • Dong Su In
    • 1
  • Hwa-Jee Chung
    • 1
    • 2
  • Jang Ryol Liu
    • 1
    • 2
  1. 1.EugentechTaejonSouth Korea
  2. 2.Korea Research Institute of Biosciences and BiotechnologyTaejonSouth Korea
  3. 3.Department of Biology Education, College of EducationChonnam National UniversityGwangju 500-757Korea

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