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Plant Cell Reports

, Volume 23, Issue 6, pp 371–376 | Cite as

Efficient plant regeneration from suspension cells of Allium cepa L.

  • Wenbo Zhang
  • Xiaofei Lin
  • Hiroyoshi Takano
  • Susumu Takio
  • Kanji Ono
Cell Biology and Morphogenesis

Abstract

Plant regeneration from calli of three cultivars of Allium cepa (Senshuki, O·Pki and Shojovaka) was investigated. Callus was induced on four variations of BDS medium containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BAP). The regeneration frequency of calli of cvs. Senshuki and O·Pki subcultured on solid MS medium supplemented with BAP ranged from 50% to 80%; this frequency decreased to less than 30% after subculture in the dark in liquid BDS medium. By repeating the dark/light transitions of the culture protocol and by selecting for green cell clusters, we were able to increase the regeneration frequency to more than 80% in all three cultivars. These cell clusters maintained a high regeneration capacity in subsequent subcultures in the absence of light for 2 months. Most (97%) of the regenerated plantlets had a normal diploid karyotype (2n=16) that was identical to that of the mother plants, although 3% of the regenerated plants of cv. Shojovaka had a tetraploid karyotype.

Keywords

Allium cepaMonocotyledon Plant regeneration Ploidy Tissue culture 

Abbreviations

BAP

6-Benzyladenine

2,4-D

2,4-Dichlorophenoxyacetic acid

Notes

Acknowledgements

This investigation was supported by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN; to H.T.).

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Wenbo Zhang
    • 1
  • Xiaofei Lin
    • 1
  • Hiroyoshi Takano
    • 1
  • Susumu Takio
    • 2
  • Kanji Ono
    • 3
  1. 1.Graduate School of Science and TechnologyKumamoto UniversityKumamotoJapan
  2. 2.Center for Marine Environment StudiesKumamoto UniversityKumamotoJapan
  3. 3.Department of Biological Science, Faculty of ScienceKumamoto UniversityKumamotoJapan

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