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Plant Cell Reports

, Volume 23, Issue 4, pp 246–250 | Cite as

Taxonomic discrimination of flowering plants by multivariate analysis of Fourier transform infrared spectroscopy data

  • S. W. Kim
  • S. H. Ban
  • H. Chung
  • S. Cho
  • H. J. Chung
  • P. S. Choi
  • O. J. Yoo
  • J. R. Liu
Physiology and Biochemistry

Abstract

Fourier transform infrared spectroscopy (FTIR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cells are analyzed. Leaf samples of seven higher plant species and varieties were subjected to FTIR to determine whether plants can be discriminated phylogenetically on the basis of biochemical profiles. A hierarchical dendrogram based on principal component analysis (PCA) of FTIR data showed relationships between plants that were in agreement with known plant taxonomy. Genetic programming (GP) analysis determined the top three to five biomarkers from FTIR data that discriminated plants at each hierarchical level of the dendrogram. Most biomarkers determined by GP analysis at each hierarchical level were specific to the carbohydrate fingerprint region (1,200–800 cm−1) of the FTIR spectrum. Our results indicate that differences in cell-wall composition and structure can provide the basis for chemotaxonomy of flowering plants.

Keywords

Dendrogram Genetic programming analysis Phylogenetic relationship Principal component analysis 

Abbreviations

FTIR

Fourier transform infrared spectroscopy

GP

Genetic programming

PCA

Principal component analysis

PyMS

Pyrolysis mass spectrometry

Notes

Acknowledgements

This work was supported by grants to JRL from the National Research Laboratory Program (M10104000234-01J000-10710), from the Strategic National R&D Program through the Genetic Resources and Information Network Center (no. BDM0100211), from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center of the Kyung Hee University funded by the Korean Ministry of Science and Technology, and from the KRIBB Research Initiative Program.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • S. W. Kim
    • 1
  • S. H. Ban
    • 1
  • H. Chung
    • 2
  • S. Cho
    • 2
  • H. J. Chung
    • 1
  • P. S. Choi
    • 3
  • O. J. Yoo
    • 4
  • J. R. Liu
    • 1
  1. 1.Laboratories of Plant Genomic Services and Plant Cell BiotechnologyKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonSouth Korea
  2. 2.Department of ChemistryHanyang UniversitySeoulSouth Korea
  3. 3.Laboratory of Functional Genomics for Plant Secondary Metabolism (National Research Laboratory)Eugentech, Inc.DaejeonSouth Korea
  4. 4.Department of Biological ScienceKorea Advanced Institute of Science and TechnologyDaejeonSouth Korea

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