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Somatic embryogenesis and plant regeneration from immature zygotic embryos of Hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.)


We established a plant regeneration system for Hinoki cypress (Chamaecyparis obtusa) via somatic embryogenesis. Embryogenic tissues were successfully induced on three kinds of Smith media from megagametophyte explants containing pre-cotyledonary embryos of C. obtusa plus-trees. Factors affecting somatic embryo maturation were examined. The concentration of polyethylene glycol 4000 in the medium was a critical factor for embryo maturation and its effective concentration was 150 g/l. The addition of 30 g/l maltose to the medium had a positive effect on embryo maturation, but sucrose was ineffective. The mature somatic embryos germinated at a germination frequency of approximately 60%, and the presence of activated charcoal was effective in stimulating plantlet growth. The plantlets acclimatized successfully in a greenhouse. To our knowledge, this is first report describing details of a plant regeneration method for C. obtusa via somatic embryogenesis.

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Fig. 1A–H
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Abscisic acid


Polyethylene glycol 4000

SM1 :

Smith Standard Embryonic Tissue Capture Medium

SM2 :

Smith Standard Embryogenesis Medium

SM3 :

Smith Embryo Develop Medium


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Correspondence to T. Taniguchi.

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Communicated by F. Sato

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Taniguchi, T., Kurita, M., Itahana, N. et al. Somatic embryogenesis and plant regeneration from immature zygotic embryos of Hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.). Plant Cell Rep 23, 26–31 (2004).

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  • Chamaecyparis obtusa
  • Somatic embryogenesis
  • Plant regeneration