Plant Cell Reports

, Volume 22, Issue 8, pp 549–552 | Cite as

Direct organogenesis of Mandevilla illustris (Vell) Woodson and effects of its aqueous extract on the enzymatic and toxic activities of Crotalus durissus terrificus snake venom

  • R. Biondo
  • A. M. Soares
  • B. W. Bertoni
  • S. C. França
  • A. M. S. Pereira
Cell Biology and Morphogenesis
First Online:
Received:
Revised:
Accepted:

Abstract

In order to produce explants of Mandevilla illustris (Vell) Woodson for the “Cerrado in vitro”, the Germplasm Bank of UNAERP, we carried out a micropropagation protocol using MS or MS/3 medium supplemented with different concentrations of 6-benzyladeninepurine (BA), Zeatin or 2-isopentenyladenine for nodal segment growth, and α-naphthaleneacetic acid, indole-3-butyric acid (IBA) or 1,4 dithiothreitol for rooting. For nodal segments, all the cytokinins tested yielded similar results. However, 2.22 µM BA is more economical to use. MS/3 medium supplemented with 0.49 µM IBA was the most appropriate medium for rooting, resulting in 29% rooted explants. The crude aqueous extract from the subterranean system (SS) of M. illustris was assayed for its inhibitory action on the enzymatic activity of Crotalus durissus terrificus snake venom, isolated basic phospholipase A2 (CB) and crotoxin. It totally inhibited the phospholipase activity of crude Cdt venom and CB toxin and inhibited the phospholipase activity of crotoxin by 49%. The toxic action of both the crude venom and crotoxin was partially inhibited—there was a prolonged survival time and a 40.0% decrease in lethality.

Keywords

Medicinal plant Micropropagation Antiophidic action Crotalus durissus terrificus Mandevilla illustrisl 

Abbreviations

BA:

6-Benzyladeninepurine

CB:

Crotalus durissus terrificus basic phospholipase A2

Cdt:

Crotalus durissus terrificus crude venom

DTT:

1,4 Dithiothreitol

IBA:

Indole-3-butyric acid

2ip:

2-Isopentenyladenine

MiHD:

Minimum indirect hemolytic dose

NAA:

α-Naphthaleneacetic acid

PBS:

Phosphate-buffered saline solution

Spermidine:

(n-[3-Aminopropyl]-1,4-butanediamine)

SS:

Subterranean system

TDZ:

Thidiazuron

Zeatin:

(6-[4-Hydroxy-3-methylbut-2-enylamino]purine)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • R. Biondo
    • 1
  • A. M. Soares
    • 1
  • B. W. Bertoni
    • 1
  • S. C. França
    • 1
  • A. M. S. Pereira
    • 1
  1. 1.Unidade de BiotecnologiaUniversidade de Ribeirão PretoRibeirão PretoBrazil

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