Plant Cell Reports

, Volume 22, Issue 5, pp 350–357 | Cite as

In vitro screening of mulberry (Morus spp.) for salinity tolerance

  • K. Vijayan
  • S. P. Chakraborti
  • P. D. Ghosh
Biotic and Abiotic Stress


An efficient in vitro screening method has been developed for mulberry (Morus spp. ) to screen salinity-tolerant genotypes from a large population. Axillary buds from field-grown plants were cultured on MS medium containing five different concentrations (0.0%, 0.25%, 0.5%, 0.75% and 1.00%) of sodium chloride (NaCl) in order to study the shoot growth pattern. Rooting was also tested at four different concentrations of NaCl (0.0%, 0.1%, 0.2% and 0.3.%). NaCl has been found to inhibit the growth and development of mulberry shoots and roots in vitro. The survivability of the axillary buds of the genotypes tested was reduced from 83.7% for the controls to 6.1% in 1.0% NaCl. The average number of roots developed by the genotypes ranged from 11.9 (controls) to 0.2 (0.3% NaCl). Out of the 63 genotypes tested, only seven—Rotundiloba, English black, Kolitha-3, Berhampore-A, Kajli, BC259 and C776—developed roots in 0.3% NaCl. Root growth was also reduced drastically from 1.8 cm for the controls to 0.1 cm in 0.3% NaCl. To test the reproducibility of the results in soil, five tolerant and two susceptible genotypes, identified in this in vitro study, were selected and tested under ex vitro conditions. The significant correlation coefficients obtained between the performances of these genotypes under both types of cultural conditions revealed that in vitro screening of mulberry through axillary bud culture is an easy and efficient method to identify salt-adapted genotypes within a limited space and time period.


Mulberry In vitro screening Axillary buds Salt tolerance 




dS m-1

Deci-Siemens per meter


Electric conductivity


α-Naphthaleneacetic acid



The authors express their sincere gratitude to Dr. B. Saratchandra, Director, CSR&TI, Berhampore and Dr. B.N. Roy, Deputy Director, MBG section, CSR&TI, Berhampore for providing the required facilities to carry out the work. Similarly, the help rendered by Dr. K.K. Das, CSR&TI, Berhampore for statistical analysis of the data is gratefully acknowledged.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • K. Vijayan
    • 1
    • 2
  • S. P. Chakraborti
    • 1
  • P. D. Ghosh
    • 1
    • 3
  1. 1.Central Sericultural Research and Training InstituteBerhamporeIndia
  2. 2.SeriBiotech RLCSB campusKodathiIndia
  3. 3.Department of BotanyUniversity of KalyaniNadiaIndia

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