Plant Cell Reports

, Volume 22, Issue 4, pp 261–267 | Cite as

A novel selection system for potato transformation using a mutated AHAS gene

  • M. AnderssonEmail author
  • A. Trifonova
  • A.-B. Andersson
  • M. Johansson
  • L. Bülow
  • P. Hofvander
Genetic Transformation and Hybridization


Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a β-glucuronidase (GUS) gene. Selection on 0.5 μM Imazamox yielded GUS expression in 93–100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.


Acetohydroxyacid synthase AHAS Herbicide tolerance Selection gene Potato transformation 



Abscisic acid


Acetohydroxyacid synthase




2, 4-Dichlorophenoxyacetic acid


Gibberellic acid


Granule bound starch synthase



MS medium

Murashige and Skoog medium


α-Naphthaleneacetic acid


Nopaline synthase


Octopine synthase


Polymerase chain reaction


5-Bromo-4-chloro-3-indolyl-beta-d-glucuronic acid


Yeast extract broth



We would like to thank the researchers at BASF Plant Science in RTP, North Carolina for the mutated AHAS gene.


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. Andersson
    • 1
    • 2
    Email author
  • A. Trifonova
    • 1
  • A.-B. Andersson
    • 1
  • M. Johansson
    • 1
  • L. Bülow
    • 2
  • P. Hofvander
    • 1
    • 3
  1. 1.Plant Science Sweden ABSvalövSweden
  2. 2.Pure and Applied BiochemistryLund UniversityLundSweden
  3. 3.Department of Plant BiologySwedish University of Agricultural SciencesUppsalaSweden

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