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Plant Cell Reports

, Volume 22, Issue 4, pp 261–267 | Cite as

A novel selection system for potato transformation using a mutated AHAS gene

  • M. AnderssonEmail author
  • A. Trifonova
  • A.-B. Andersson
  • M. Johansson
  • L. Bülow
  • P. Hofvander
Genetic Transformation and Hybridization

Abstract

Acetohydroxyacid synthase (AHAS) is the target enzyme for a number of herbicides. A S653N mutation in the AHAS gene results in an increased tolerance to imidazolinone herbicides. We have investigated the use of the mutated gene as selection gene for potato transformation. This resulted in a transformation system with a very high transformation frequency and low rate of escapes. The mutated AHAS gene was introduced into transformed potato together with a β-glucuronidase (GUS) gene. Selection on 0.5 μM Imazamox yielded GUS expression in 93–100% of regenerated shoots. Furthermore the mutated AHAS gene was used as selection gene for production of high-amylopectin potato lines. The high transformation frequency was verified and potato lines with the desirable starch quality were obtained.

Keywords

Acetohydroxyacid synthase AHAS Herbicide tolerance Selection gene Potato transformation 

Abbreviations

ABA

Abscisic acid

AHAS

Acetohydroxyacid synthase

BAP

6-Benzylaminopurine

2,4-D

2, 4-Dichlorophenoxyacetic acid

GA3

Gibberellic acid

GBSS

Granule bound starch synthase

GUS

β-Glucuronidase

MS medium

Murashige and Skoog medium

NAA

α-Naphthaleneacetic acid

nos

Nopaline synthase

OCS

Octopine synthase

PCR

Polymerase chain reaction

X-gluc

5-Bromo-4-chloro-3-indolyl-beta-d-glucuronic acid

YEB

Yeast extract broth

Notes

Acknowledgement

We would like to thank the researchers at BASF Plant Science in RTP, North Carolina for the mutated AHAS gene.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. Andersson
    • 1
    • 2
    Email author
  • A. Trifonova
    • 1
  • A.-B. Andersson
    • 1
  • M. Johansson
    • 1
  • L. Bülow
    • 2
  • P. Hofvander
    • 1
    • 3
  1. 1.Plant Science Sweden ABSvalövSweden
  2. 2.Pure and Applied BiochemistryLund UniversityLundSweden
  3. 3.Department of Plant BiologySwedish University of Agricultural SciencesUppsalaSweden

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