Plant Cell Reports

, Volume 22, Issue 2, pp 150–158 | Cite as

Expressed sequence tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]

  • C. S. Jang
  • J. Y. Kim
  • J. W. Haam
  • M. S. Lee
  • D. S. Kim
  • Y. W. Li
  • Y. W. Seo
Genetics and Genomics

Abstract

Of the 16 known biotypes of the Hessian fly [Mayetiola destructor (Say)], biotype L is recognized as being the most virulent. We have previously reported the development of near-isogenic lines (NILs) (BC3F3:4) by backcross introgression (Coker797*4/Hamlet) that differed by the presence or absence of the H21 gene on 2RL chromatin. Florescence in situ hybridization analysis revealed introgressed 2RLs in NILs possessing the H21 gene, but no signal was detected in NILs lacking 2RL. As part of an approach to elucidate molecular interactions between plants and the Hessian fly, a cDNA library from NILs with H21 infested by larvae of biotype L of the Hessian fly was constructed for expressed sequence tag (EST) analysis. Of 1,056 sequenced reactions attempted, 919 ESTs produced some lengths of readable sequences. Based on their putative identification, 730 ESTs that showed significant similarity with amino acid sequences registered in the gene bank were divided into 13 functional categories. Defense- and stress-related genes represented about 16.1%, including protease inhibition, oxidative burst, lignin synthesis, and phenylpropanoid metabolism. EST clones obtained from the cDNA library may provide a clue to the molecular interactions between plant and larva of the Hessian fly larval infestation.

Keywords

2RL H21 Hessian fly EST Wheat-rye translocation 

Abbreviations

ESTs

Expressed sequence tags

FISH

Florescence in situ hybridization

NILs

Near-isogenic lines

Notes

Acknowledgements

The authors extend special thanks to Dr. Robert A. Graybosch (USDA-ARS, University of Nebraska-Lincoln) for his thoughtful comments and suggestions on the manuscript and Drs. Rogers H. Ratcliffel and Sue E. Cambron (University of Purdue) for providing Hessian fly stocks. This work was supported by a grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea. It was also partially funded by a Korea University Grant.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • C. S. Jang
    • 1
  • J. Y. Kim
    • 1
  • J. W. Haam
    • 1
  • M. S. Lee
    • 1
  • D. S. Kim
    • 1
  • Y. W. Li
    • 2
  • Y. W. Seo
    • 1
  1. 1.Department of Crop Science, Division of Biotechnology and Genetic EngineeringKorea UniversitySeoulKorea
  2. 2.State Key Laboratory of Plant Cell Chromosome Engineering, Institute of Genetics and Developmental BiologyThe Chinese Academy of SciencesBeijingChina

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