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Plant Cell Reports

, Volume 21, Issue 5, pp 408–414 | Cite as

Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.)

  •  J. Mithila
  •  J. Hall
  • J.M.R. Victor
  •  P. Saxena
Cell Biology and Morphogenesis

Abstract.

Regeneration via shoot organogenesis and somatic embryogenesis was observed from thidiazuron (TDZ)-treated leaf and petiole explants of greenhouse- and in vitro-grown African violet plants. The response of cultures to other growth regulators over a range of 0.5 µM to 10 µM was 50% less than that observed with TDZ. A comparative study among several cultivars of African violet indicated that 'Benjamin' and 'William' had the highest regeneration potential. In 'Benjamin', higher frequencies of shoot organogenesis (twofold) and somatic embryogenesis (a 50% increase) were observed from in vitro- and greenhouse-grown plants, respectively. At concentrations lower than 2.5 µM, TDZ induced shoot organogenesis, whereas at higher doses (5–10 µM) somatic embryos were formed. These findings provide the first report of simultaneous shoot organogenesis and somatic embryogenesis of African violet explants in response to TDZ.

Saintpaulia ionantha Wendl. Morphogenesis Regeneration Auxin Cytokinin Thidiazuron 

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  •  J. Mithila
    • 1
  •  J. Hall
    • 1
  • J.M.R. Victor
    • 2
  •  P. Saxena
    • 3
  1. 1.Department of Environmental Biology, University of Guelph, Ontario, Canada N1G 2W1
  2. 2.International Paper Corp, Bainbridge, Georgia, USA
  3. 3.Department of Plant Agriculture, University of Guelph, Ontario, Canada N1G 2W1

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