Use of representational difference analysis for the characterization of sequence differences between date palm varieties
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Representational difference analysis was applied to subtract the genomes of the two date palm varieties, Barhee and Medjool, for identification and characterization of unique genome differences suitable for discriminating between individual plants and the two varieties. Three different DNA difference products were isolated from Barhee representing families of dispersed, repeated variable sequences present in the genome of both varieties. Several variant members of repeated DNA were detected by sequence analysis, containing base changes from C to T and G to A and short deletions. Mutated DNA sequences could be amplified in a polymerase chain reaction-based test from a much smaller number of Barhee plants than from Medjool plants allowing the differentiation between individual plants and partial discrimination between varieties.
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