Plant Cell Reports

, Volume 21, Issue 2, pp 147–152

Production of fertile intergeneric somatic hybrids between Brassica napus and Sinapis arvensis for the enrichment of the rapeseed gene pool

  •  Q. Hu
  •  S. Andersen
  •  C. Dixelius
  •  L. Hansen
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-002-0491-7

Cite this article as:
Hu, Q., Andersen, S., Dixelius, C. et al. Plant Cell Rep (2002) 21: 147. doi:10.1007/s00299-002-0491-7

Abstract.

Somatic hybrids between Brassica napus (oilseed rape) and its wild relative Sinapis arvensis (Xinjiang wild mustard) from northwestern China were produced by fusing mesophyll protoplasts. Fifty-four plants were identified as symmetric hybrids and four as asymmetric hybrids by random amplified polymorphic DNA analysis and nuclear DNA content. The morphology of investigated 58 hybrid plants resembled characters from both parental species. Highly fertile hybrids were recovered where the fertility was associated with the choice of B. napus genotype. Enhanced disease resistance to Leptosphaeria maculans was found in S. arvensis and in the hybrid offspring. This plant material has great potentials not only for use as a bridge for the introduction of a number of valuable traits from the wild species to Brassica crops but also for breeding new varieties with improved blackleg resistance.

Blackleg resistance Intergeneric hybridization Protoplast fusion Xinjiang wild mustard 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  Q. Hu
    • 1
  •  S. Andersen
    • 1
  •  C. Dixelius
    • 3
  •  L. Hansen
    • 1
  1. 1.Department of Agricultural Sciences, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, 1871 Frederiksberg C, Denmark
  2. 2.Institute of Oil Crops, Chinese Academy of Agricultural Sciences, 430062 Wuhan, P.R. China
  3. 3.Department of Plant Biology, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007 Uppsala, Sweden
  4. 4.Present address: Department of Plant Biology, Swedish University of Agricultural Sciences P.O. Box 7080, 75007 Uppsala, Sweden

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