Advertisement

Plant Cell Reports

, Volume 20, Issue 8, pp 685–690 | Cite as

Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals

  •  M. Zheng
  •  W. Liu
  •  Y. Weng
  •  E. Polle
  •  C. Konzak
Cell Biology and Morphogenesis

Abstract.

Microspores were isolated from wheat (Triticum aestivum L.) spikes by means of a micro-blender immediately following their removal from the donor plants. Isolated microspores were then subjected to a chemical treatment consisting of 0.18 mM 2-hydroxynicotinic acid, minerals and 9% maltose in the dark at 25°C for 38–52 h. Following purification via filtration and gradient centrifugation on 21% maltose, the microspores were cultured in the presence of excised ovaries in liquid medium at 27°C. Embryoid yield, percentage of green plants, and the frequency of spontaneously doubled haploids ranged from 360 to 4,914 embryoids per spike, 15% to 95%, and 39% to 78%, respectively. Other compounds that were effective in maintaining viability and triggering microspore embryogenesis were benzotriazole-5-carboxylic acid and violuric acid monohydrate. This system has proven to be highly efficient for producing doubled haploids over a range of genotypes.

Androgenesis Doubled haploid Embryoid 2-Hydroxynicotinic acid Wheat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  M. Zheng
    • 1
  •  W. Liu
    • 1
  •  Y. Weng
    • 1
  •  E. Polle
    • 1
  •  C. Konzak
    • 1
  1. 1.Northwest Plant Breeding Co, 2001 Country Club Rd, Pullman, WA 99163, USA

Personalised recommendations