Rheumatology International

, Volume 35, Issue 3, pp 471–477 | Cite as

Serum IL-33 level and IL-33 gene polymorphisms in Behçet’s disease

  • Suleyman Serdar KocaEmail author
  • Murat Kara
  • Firat Deniz
  • Metin Ozgen
  • Caner Feyzi Demir
  • Nevin Ilhan
  • Ahmet Isik
Original Article - Genes and Disease


Behçet’s disease (BD) is a chronic inflammatory disease. Increased productions of cytokines including interleukin (IL)-1β and IL-18 are documented, and IL-1α and β gene polymorphisms are associated with susceptibility to the disease. IL-33 is a recently discovered member of IL-1 cytokine family. The aim of the study was to detect serum IL-33 level and IL-33 gene polymorphisms in a cohort of BD. Unrelated 117 patients with BD and 149 healthy controls (HC) were enrolled. Serum IL-33 levels were analyzed by enzyme-linked immunosorbent assay method. DNA samples were harvested using an appropriate commercial DNA isolation kit. Four single nucleotide polymorphisms of IL-33 gene (rs7044343, rs1157505, rs11792633 and rs1929992) were genotyped using the appropriate commercial primer/probe sets on real-time PCR. Serum IL-33 level was not significantly different in the BD and HC groups (p > 0.05). However, its level was lower in the active BD patients compared to the inactive ones and HC group (p = 0.044 and p = 0.037, respectively). There was no significant difference in terms of the genotypic and allelic distributions of rs1157505 and rs1929992 polymorphisms (p > 0.05 for all). However, the TT variants of rs7044343 and rs11792633 polymorphisms were very rare, and the T allele frequencies of these polymorphisms were lower, in the BD group compared to the HC group (p < 0.0001 for all). The rs7044343 and rs11792633 variants of IL-33 gene are associated with the decreased risk of BD in our cohort. Therefore, it may be concluded that IL-33 acts a protective role on the pathogenesis of BD.


Behçet’s disease IL-33 Polymorphisms 


Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Suleyman Serdar Koca
    • 1
    Email author
  • Murat Kara
    • 2
  • Firat Deniz
    • 3
  • Metin Ozgen
    • 4
  • Caner Feyzi Demir
    • 5
  • Nevin Ilhan
    • 6
  • Ahmet Isik
    • 1
  1. 1.Department of Rheumatology, Faculty of MedicineFirat UniversityElazigTurkey
  2. 2.Department of Medical Genetics, Faculty of MedicineMugla Sitki Kocman UniversityMuglaTurkey
  3. 3.Department of Internal Medicine, Faculty of MedicineFirat UniversityElazigTurkey
  4. 4.Department of Rheumatology, Faculty of Medicine19 Mayis UniversityMalatyaTurkey
  5. 5.Department of Neurology, Faculty of MedicineFirat UniversityElazigTurkey
  6. 6.Department of Biochemistry, Faculty of MedicineFirat UniversityElazigTurkey

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