Rheumatology International

, Volume 31, Issue 7, pp 865–869 | Cite as

Anti-annexin II antibody is associated with thrombosis and/or pregnancy morbidity in antiphospholipid syndrome and systemic lupus erythematosus with thrombosis

  • Wen Ao
  • Hui Zheng
  • Xiao-Wei Chen
  • Yan Shen
  • Cheng-De Yang
Original Article


This study investigated the prevalence of antibody against annexin II (A2) in patients with antiphospholipid syndrome (APS) and systemic lupus erythematosus (SLE). It also explored the role of this antibody in thrombosis. Purified recombinant A2 was used in ELISA to measure the levels of IgG anti-A2 antibody in 101 APS patients, 41 SLE patients with thrombosis, 124 SLE patients without thrombosis, and 120 healthy controls. The positive rates of IgG anti-A2 antibody in APS patients and SLE patients with thrombosis were 21.8 and 26.8%, respectively. Both values are significantly higher than that in SLE patients without thrombosis (6.5%). IgG anti-A2 antibody is associated with thrombosis and/or pregnancy morbidity (P < 0.001). The data show that the levels of anti-A2 antibody are higher in APS patients and SLE patients with thrombosis. Further, the presence of this antibody is associated with thrombosis and/or pregnancy morbidity, suggesting that anti-A2 antibody may be helpful in identifying potential APS cases.


Anti-annexin II antibody Antiphospholipid antibodies Thrombosis Antiphospholipid syndrome 


  1. 1.
    Levine JS, Branch DW, Rauch J (2002) The antiphospholipid syndrome. N Engl J Med 346:752–763. doi:10.1056/NEJMra002974 PubMedCrossRefGoogle Scholar
  2. 2.
    Merrill JT (2004) Antibodies and clinical features of the antiphospholipid syndrome as criteria for systemic lupus erythematosus. Lupus 13:869–876. doi:10.1191/0961203304lu2026oa PubMedCrossRefGoogle Scholar
  3. 3.
    Miyakis S, Lockshin MD, Atsumi T et al (2006) International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost 4:295–306. doi:10.1111/j.1538-7836.2006.01753.x PubMedCrossRefGoogle Scholar
  4. 4.
    Cugno M, Cabibbe M, Galli M et al (2004) Antibodies to tissue-type plasminogen activator (t-PA) in patients with antiphospholipid syndrome: evidence of interaction between the antibodies and the catalytic domain of tPA in 2 patients. Blood 103:2121–2126. doi:10.1182/blood-2003-07-2422 PubMedCrossRefGoogle Scholar
  5. 5.
    Lu CS, Horizon AA, Hwang KK et al (2005) Identification of polyclonal and monoclonal antibodies against tissue-type plasminogen activator in the antiphospholipid syndrome. Arthritis Rheum 52:4018–4027. doi:10.1002/art.21485 PubMedCrossRefGoogle Scholar
  6. 6.
    Emans N, Gorvel JP, Walter C et al (1993) Annexin II is a major component of fusogenic endosomal vesicles. J Cell Biol 120:1357–1369. doi:10.1083/jcb.120.6.1357 PubMedCrossRefGoogle Scholar
  7. 7.
    Futter CE, White IJ (2007) Annexins and endocytosis. Traffic 8:951–958. doi:10.1111/j.1600-0854.2007.00590.x PubMedCrossRefGoogle Scholar
  8. 8.
    Kirshner J, Schumann D, Shively JE (2003) CEACAM1, a cell-cell adhesion molecule, directly associates with annexin II in a three-dimensional model of mammary morphogenesis. J Biol Chem 278:50338–50345. doi:10.1074/jbc.M309115200 PubMedCrossRefGoogle Scholar
  9. 9.
    Gerke V, Moss SE (2002) Annexins: from structure to function. Physiol Rev 82:331–371PubMedGoogle Scholar
  10. 10.
    Dreier R, Schmid KW, Gerke V, Riehemann K (1998) Differential expression of annexins I, II and IV in human tissues: an immunohistochemical study. Histochem Cell Biol 110:137–148. doi:10.1007/s004180050275 PubMedCrossRefGoogle Scholar
  11. 11.
    Filipenko NR, Waisman DM (2001) The C terminus of annexin II mediates binding to F-actin. J Biol Chem 276:5310–5315. doi:10.1074/jbc.M009710200 PubMedCrossRefGoogle Scholar
  12. 12.
    Hajjar KA, Jacovina AT, Chacko J (1994) An endothelial cell receptor for plasminogen/tissue plasminogen activator. I. Identity with annexin II. J Biol Chem 269:21191–21197PubMedGoogle Scholar
  13. 13.
    Kassam G, Choi KS, Ghuman J et al (1998) The role of annexin II tetramer in the activation of plasminogen. J Biol Chem 273:4790–4799. doi:10.1074/jbc.273.8.4790 PubMedCrossRefGoogle Scholar
  14. 14.
    Yang CD, Hwang KK, Yan W et al (2004) Identification of anti-plasmin antibodies in the antiphospholipid syndrome that inhibit degradation of fibrin. J Immunol 172:5765–5773PubMedGoogle Scholar
  15. 15.
    Hochberg MC (1997) Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 40:1725. doi:10.1002/art.1780400928 PubMedCrossRefGoogle Scholar
  16. 16.
    Khamashta MA, Cuadrado MJ, Mujic F, Taub NA, Hunt BJ, Hughes GR (1995) The management of thrombosis in the antiphospholipid-antibody syndrome. N Engl J Med 332:993–997. doi:10.1056/NEJM199504133321504 PubMedCrossRefGoogle Scholar
  17. 17.
    Branch DW, Khamashta MA (2003) Antiphospholipid syndrome: obstetric diagnosis, management and controversies. Obstet Gynecol 101:1333–1344. doi:10.1016/S0029-7844(03)00363-6 PubMedCrossRefGoogle Scholar
  18. 18.
    Bertolaccini ML, Gomez S, Pareja JF et al (2005) Antiphospholipid antibody tests: spreading the net. Ann Rheum Dis 64:1639–1643. doi:10.1136/ard.2005.035824 PubMedCrossRefGoogle Scholar
  19. 19.
    Tanaka Y, Ishii H, Hiraoka M et al (2007) Efficacy of recombinant annexin 2 for fibrinolytic therapy in a rat embolic stroke model: a magnetic resonance imaging study. Brain Res 1165:135–143. doi:10.1016/j.brainres.2007.06.039 PubMedCrossRefGoogle Scholar
  20. 20.
    Cesarman-Maus G, Rios-Luna NP, Deora AB et al (2006) Autoantibodies against the fibrinolytic receptor, annexin 2, in antiphospholipid syndrome. Blood 107:4375–4382. doi:10.1182/blood-2005-07-2636 PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Wen Ao
    • 1
  • Hui Zheng
    • 1
  • Xiao-Wei Chen
    • 1
  • Yan Shen
    • 1
  • Cheng-De Yang
    • 1
  1. 1.Department of RheumatologyRenji Hospital, Shanghai Jiaotong University School of MedicineShanghaiChina

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