Rheumatology International

, Volume 30, Issue 8, pp 1025–1033 | Cite as

Differential effect of IL-1β and TNF-α on the production of IL-6, IL-8 and PGE2 in fibroblast-like synoviocytes and THP-1 macrophages

  • Hyun Mi Choi
  • Da Hee Oh
  • Jun Soo Bang
  • Hyung-In Yang
  • Myung Chul YooEmail author
  • Kyoung Soo KimEmail author
Original Article


Inflammation in the joint of rheumatoid arthritis is a complex immune reaction facilitated by various factors, such as cytokines, cells and hypoxia. Thus, we evaluated their relative capacity to produce proinflammatory mediators in response to IL-1β, TNF-α or IL-17 under hypoxia or normoxia in fibroblast-like synoviocytes (FLSs) and macrophages. The level of IL-6 expression was strongly increased in both FLSs and THP-1 macrophages in response to IL-1β and TNF-α, but the level by TNF-α was less than that by IL-1β. In contrast, the expression of IL-8 in both cell types was strongly stimulated by both IL-1β and TNF-α. In FLSs, PGE2 production increased only in response to IL-1β; and no effect was observed in THP-1 cells and TNF-α-stimulated FLSs. In addition, the production by IL-17 was extremely low when compared with those induced by IL-1β or TNF-α in FLSs and THP-1 cells. Hypoxia (2% O2) decreased IL-1β-stimulated production of PGE2, even though it increased the expression of mRNA and protein of COX-2. These results suggest that IL-1β and TNF-α differentially regulate gene expression in FLSs and macrophages under hypoxia or normoxia.


Fibroblast-like synoviocyte Macrophage Rheumatoid arthritis COX-2 TNF-α IL-1β Hypoxia 



This work was supported by a research grant from the Korean Ministry of Health and Welfare (03-PJ9-PG6-SO01-002).


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.East-West Neo Medical Center, East-West Bone and Joint Research InstituteKyung Hee UniversitySeoulRepublic of Korea
  2. 2.Department of Orthopedic Surgery, East-West Neo Medical CenterKyung Hee UniversitySeoulRepublic of Korea

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