Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet’s disease etiopathogenesis
- 142 Downloads
The objective of this study was to investigate the expressions of toll-like receptors on neutrophils and monocytes in Behcet’s disease (BD). Forty-two patients with BD were included in the study. Baseline and stimulated cells with heat shock protein-60 (HSP-60), lipopolysaccharide (LPS) and crude extract of Streptococcus sanguis (SS) were analyzed for toll-like receptor-1, -2, -4 and -6 expressions using flow cytometer. Results were confirmed using semi-quantitative PCR technique. The mean frequency of TLR-6 expressing granulocytes in BD patients was significantly lower than in controls (4.2 ± 0.6 vs. 9.2 ± 0.6 and 8.0 ± 1.4, BD vs. rheumatoid arthritis (RA) and healthy control (HC); P < 0.05). TLR-6 expressing granulocyte population was enhanced after stimulation with HSP-60 and SS (20 ± 0.5 and 12.8 ± 0.6, respectively). Decreased TLR-2 expression was noted in monocytes of BD patients after stimulation with HSP-60 and LPS (71.0 ± 0.5 and 73.0 ± 0.5; P < 0.02, for both antigens). Functional abnormalities of these receptors or different activation cascades by different microorganisms are associated with disease pathogenesis in Behcet’s disease.
KeywordsBehcet’s disease Innate immunity cells Toll-like receptors
- 1.Yazici H, Yurdakul S, Hamuryudan V (1998) Behcet’s syndrome. In: Klippel JH, Dieppe PA (eds) Rheumatology. Mosby, London, 7:26:1–6Google Scholar
- 2.Mumcu G, Inanc N, Yavuz S, Direskeneli H (2007). The role of infectious agents in the pathogenesis, clinical manifestations and treatment strategies in Behcet’s disease. Clin Exp Rheumatol, Sept. 2007 SupplementGoogle Scholar
- 12.International study group for Behcet’s disease (1990) Criteria for diagnosis of Behcet’s disease. Lancet 335:1078–1080Google Scholar