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Rheumatology International

, Volume 28, Issue 5, pp 401–406 | Cite as

Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet’s disease etiopathogenesis

  • Sule Yavuz
  • Yesim Elbir
  • Aysin Tulunay
  • Emel Eksioglu-Demiralp
  • Haner Direskeneli
Original Article

Abstract

The objective of this study was to investigate the expressions of toll-like receptors on neutrophils and monocytes in Behcet’s disease (BD). Forty-two patients with BD were included in the study. Baseline and stimulated cells with heat shock protein-60 (HSP-60), lipopolysaccharide (LPS) and crude extract of Streptococcus sanguis (SS) were analyzed for toll-like receptor-1, -2, -4 and -6 expressions using flow cytometer. Results were confirmed using semi-quantitative PCR technique. The mean frequency of TLR-6 expressing granulocytes in BD patients was significantly lower than in controls (4.2 ± 0.6 vs. 9.2 ± 0.6 and 8.0 ± 1.4, BD vs. rheumatoid arthritis (RA) and healthy control (HC); P < 0.05). TLR-6 expressing granulocyte population was enhanced after stimulation with HSP-60 and SS (20 ± 0.5 and 12.8 ± 0.6, respectively). Decreased TLR-2 expression was noted in monocytes of BD patients after stimulation with HSP-60 and LPS (71.0 ± 0.5 and 73.0 ± 0.5; P < 0.02, for both antigens). Functional abnormalities of these receptors or different activation cascades by different microorganisms are associated with disease pathogenesis in Behcet’s disease.

Keywords

Behcet’s disease Innate immunity cells Toll-like receptors 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Sule Yavuz
    • 1
    • 3
  • Yesim Elbir
    • 2
  • Aysin Tulunay
    • 2
  • Emel Eksioglu-Demiralp
    • 2
  • Haner Direskeneli
    • 1
  1. 1.Department of RheumatologyUniversity of MarmaraIstanbulTurkey
  2. 2.Department of Haematology–ImmunologyUniversity of MarmaraIstanbulTurkey
  3. 3.IstanbulTurkey

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