Rheumatology International

, Volume 24, Issue 2, pp 71–76 | Cite as

Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

  • Samuel Solomon
  • Madhan Masilamani
  • Subhasis Mohanty
  • Jörg E. Schwab
  • Eva-Maria Boneberg
  • Harald IllgesEmail author
Original Article


Using single cell suspensions from synovial fluid cells of arthritis patients, we observed differentiation of three-dimensional tissues in vitro. This new model of pannus-like tissue (PLT) might be useful to study pannus tissue formation and differentiation. In the PLT cultures, we observed two cell types, fibroblast-like and macrophage-like cells, defined by their distinct morphology and major histocompatibility complex (MHC) by human leukocyte antigen (HLA) class II expression. We could discriminate several intermediate steps of differentiation which finally led to 3D villi-like structures. Secretion of interferon gamma, interleukin-10, and tumor necrosis factor alpha was measured in the culture supernatants. Using methotrexate at various concentrations, the growth of PLT could be inhibited. We describe definite intermediate steps of differentiation. The present approach could be a suitable model for the in vitro study of pannus tissue formation.


Cytokine Fibroblast In vitro tissue Monocyte Pannus 



This work was supported through BMBF grant 01GG9834/1and the 3R Foundation to HI. The PLT system is protected by patent PCT/EP97/04308, Nr. 97.938885.7.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Samuel Solomon
    • 1
  • Madhan Masilamani
    • 1
  • Subhasis Mohanty
    • 1
  • Jörg E. Schwab
    • 1
  • Eva-Maria Boneberg
    • 2
  • Harald Illges
    • 1
    • 2
    Email author
  1. 1.Immunology, Department of Biology, Faculty of SciencesUniversity of KonstanzKonstanzGermany
  2. 2. Biotechnology Institute ThurgauTägerwilenSwitzerland

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