Rheumatology International

, Volume 21, Issue 5, pp 182–188 | Cite as

CD69 expression on lymphocytes and interleukin-15 levels in synovial fluids from different inflammatory arthropathies

  • Ana M. Ortiz
  • Armando Laffon
  • Isidoro Gonzalez-Alvaro
Original Article

Abstract

Objective. The aim was to study a possible relationship between CD69 expression on lymphocytes and interleukin-15 (IL-15) levels in synovial fluid (SF) from patients with different inflammatory arthropathies. Methods. CD69 expression was assessed by two-color flow cytometry on different subsets of synovial fluid lymphocytes (SFL) obtained from patients with diagnoses of rheumatoid arthritis (RA), seronegative spondyloarthropathy (SSd), and crystal-associated arthritis (CAA). The IL-15 levels in synovial fluid supernatants were measured by enzyme-linked immunoassay (ELISA). Results. No significant differences between the three groups of arthropathies were observed in the distribution of synovial fluid lymphocyte subsets. CD69-positive SFL were mainly CD3-, CD45RO-, and CCR5-positive cells. Although no significant differences in the percentage of CD69-positive lymphocytes were observed between the three groups of patients, the highest level of CD69 expression on lymphocytes was observed in RA patients (mean fluorescence intensity 37.9±5.2 compared to 17.5±3.3 in SSd and 12.4±2.6 in CAA, P=0.007 and P<0.001, respectively). In addition, the expression of CD69 on SFL from RA correlated with their respective IL-15 levels measured in SF supernatants. Conclusion. Our data provide in vivo evidence of the putative role that IL-15 can play in the high expression of CD69 on SFL from RA patients.

IL-15 CD69 CCR5 Rheumatoid arthritis Spondyloarthropathies 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Ana M. Ortiz
    • 1
  • Armando Laffon
    • 1
  • Isidoro Gonzalez-Alvaro
    • 1
  1. 1.Rheumatology Department, Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, C/Diego de León no. 62, 28006 Madrid, SpainSpain

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