Current Genetics

, Volume 40, Issue 3, pp 186–194 | Cite as

Secondary DNA structure analysis of the coding strand switch regions of five Leishmania major Friedlin chromosomes

  • Valentina Tosato
  • Laura Ciarloni
  • Alasdair C. Ivens
  • Marie-Adèle Rajandream
  • Bart G. Barrell
  • Carlo V. Bruschi
Research Article

Abstract.

As part of the EULEISH international genome project, a region of 74,674 nucleotides from chromosome 21 of Leishmania major Friedlin was subcloned and sequenced; and 31 new coding sequences were predicted. Of particular interest was a unique coding strand switching region covering 1.6 kb of DNA; and this was subjected to further investigation. Bioinformatic analysis of this region revealed an unusually high AT composition, a lack of putative hairpins and a strong curvature of the DNA in agreement with the structural characteristics of similar regions of other Leishmania chromosomes. These observations and a comparison with the secondary DNA structure of four other Leishmania chromosomes and chromosomes of different organisms could suggest a functional role of this region in transcription and mitotic division.

Leishmania major Friedlin genome Switching point DNA secondary structures Bioinformatic analysis 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Valentina Tosato
    • 2
  • Laura Ciarloni
    • 2
  • Alasdair C. Ivens
    • 3
  • Marie-Adèle Rajandream
    • 3
  • Bart G. Barrell
    • 3
  • Carlo V. Bruschi
    • 1
  1. 1.Microbiology Group, International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, 34012 Trieste, Italy
  2. 2.Department of Biology, Genomics Group, University of Trieste, Trieste, 34148, Italy
  3. 3.The Sanger Centre, Hinxton, Cambridgeshire, CB10 1SA, UK

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