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Current Genetics

, Volume 39, Issue 3, pp 127–136 | Cite as

A novel putative reductase (Cpd1p) and the multidrug exporter Snq2p are involved in resistance to cercosporin and other singlet oxygen-generating photosensitizers in Saccharomyces cerevisiae

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Original Paper

Abstract.

Phytopathogenic Cercospora species produce cercosporin, a photoactivated perylenequinone toxin that belongs to a family of photosensitizers which absorb light energy and produce extremely cytotoxic, reactive oxygen species. In this work, we used Saccharomyces cerevisiae as a model system for the identification and cloning of genes whose products mediate cercosporin detoxification. Two genesexpressed in high-copy number vectors conferred cercosporin resistance to an otherwise sensitive strain. One gene codes for Snq2p, a well-characterized multidrug, ABC-type, efflux protein. The other, designated CPD1 (Cercosporin Photosensitizer Detoxification), encodes a novel protein with significant similarity to the FAD-dependent pyridine nucleotide reductases. We showed that over-expression of either of these proteins can also mediate resistance to other singlet oxygen-generating compounds. The involvement of Snq2p and Cpd1p in photosensitizer detoxification reinforces previous observations which suggested that singlet oxygen acts on membrane lipids and that cellular resistance to cercosporin is mediated by a mechanism involving toxin efflux and/or toxin reduction.

Cercosporin detoxification Singlet oxygen-resistance Gene overexpression Multidrug resistance 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

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  1. 1.Department of Crop Science, Plant Biochemistry & Biotechnology Laboratory, School of Agricultural Technology, Technological Educational Institute, P.O. Box 140, 71110 Heraklion, Crete, Greece
  2. 2.Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, P.O. Box 1527, 71110 Heraklion, Crete, Greece
  3. 3.Department of Biology, University of Athens, Panepistimioupolis, 15781 Athens, Greece
  4. 4.Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece

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