Current Genetics

, Volume 36, Issue 5, pp 262–269 | Cite as

A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy

  • Oleh V. Stasyk
  • Ida J. van der Klei
  • Anna Rita Bellu
  • Shigang Shen
  • Jan A. K. W. Kiel
  • James M. Cregg
  • Marten Veenhuis
ORIGINAL PAPER

Abstract

Methylotrophic yeasts contain large peroxisomes during growth on methanol. Upon exposure to excess glucose or ethanol these organelles are selectively degraded by autophagy. Here we describe the cloning of a Pichia pastoris gene (PpVPS15) involved in peroxisome degradation, which is homologous to Saccharomyces cerevisiae VPS15. In methanol-grown cells of a P. pastoris VPS15 deletion strain, the levels of peroxisomal marker enzymes remained high after addition of excess glucose or ethanol. Electron microscopic studies revealed that the organelles were not taken up by vacuoles, suggesting that PpVPS15 is required at an early stage in peroxisome degradation.

Key wordsPichia pastoris Peroxisome degradation Autophagy VPS15 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Oleh V. Stasyk
    • 2
  • Ida J. van der Klei
    • 1
  • Anna Rita Bellu
    • 1
  • Shigang Shen
    • 2
  • Jan A. K. W. Kiel
    • 1
  • James M. Cregg
    • 2
  • Marten Veenhuis
    • 1
  1. 1.Eukaryotic Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), Biological Centre, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands e-mail: M.Veenhuis@biol.rug.nl Tel.: +1-50-363 2176 Fax: +1-50-363 5205NL
  2. 2.Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, P.O. Box 91000, Portland, OR 97291-1000, USAUS

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