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Current Genetics

, Volume 35, Issue 1, pp 36–40 | Cite as

Cloning and characterization of pl1 encoding an in planta-secreted pectate lyase of Fusarium oxysporum

  • M. Dolores Huertas-González
  • M. Carmen Ruiz-Roldán
  • Fe I. García Maceira
  • M. Isabel G. Roncero
  • A. Di Pietro
ORIGINAL PAPER

Abstract

A pectate lyase (PL1) from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici was previously characterized, and evidence was obtained for its production in planta. The gene encoding PL1 was isolated from a genomic library of F. oxysporum f.sp. lycopersici. Pl1 encodes a 240 amino-acid polypeptide with one putative N-glycosylation site and a 15 amino-acid N-terminal signal peptide. PL1 showed 89%, 67%, 55% and 56% identity with the products of the Fusarium solani f.sp. pisi pelA, pelB, pelC and pelD genes, respectively. A single copy of the gene was detected in different formae speciales of F. oxysporum. The pl1 transcript was observed during growth on polygalacturonic acid sodium salt and tomato vascular tissue, but not on pectin or glucose. RT-PCR showed pl1 expression in roots and stems of tomato plants infected by F. oxysporum f.sp. lycopersici.

Key wordsFusarium oxysporum In planta expression Pathogenicity factor Pectate lyase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. Dolores Huertas-González
    • 1
  • M. Carmen Ruiz-Roldán
    • 1
  • Fe I. García Maceira
    • 1
  • M. Isabel G. Roncero
    • 1
  • A. Di Pietro
    • 1
  1. 1.Departamento de Genética, Facultad de Ciencias, Avda. de S. Alberto Magno S/N, 14071 Córdoba, Spain e-mail: ge2dipia@uco.es Tel.: +34-57218601 Fax: +34-57218606ES

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